Nitroreductase from Escherichia coli
SIGMA/N9284 - ≥90% (SDS-PAGE), recombinant, expressed in E. coli
Synonym: NTRA
MDL Number: MFCD03457727
Product Type: Chemical
SDS-PAGE Nitroreductase from Escherichia coli (Cat. No. N9284) was assessed on SDS-PAGE.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
12 Principles of Green Chemistry: Principle 5—Safer Solvents and Auxiliaries. This product utilizes reduced volumes of auxiliary substances that are less hazardous.
| assay | ≥90% (SDS-PAGE) |
| form | lyophilized powder |
| greener alternative category |  , Re-engineered |
| greener alternative product characteristics | Waste Prevention Safer Solvents and Auxiliaries Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| mol wt | monomer 24000 |
| Quality Level | 200 |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| specific activity | ≥100 units/mL |
| storage temp. | −20°C |
| sustainability | Greener Alternative Product |
| UniProt accession no. | P38489  |
| Application: | Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes. |
| Biochem/physiol Actions: | Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954. |
| Biochem/physiol Actions: | Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical. |
| Biochem/physiol Actions: | Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant. |
| Biochem/physiol Actions: | Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. |
| General description: | Lyophilized powder containing PBS. Does not contain BSA as excipient |
| General description: | Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme. |
| Other Notes: | One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C. |
| Preparation Note: | Produced using animal component-free materials. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥100 units/mL |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |