Anti-Neuropeptide Y (NPY) antibody produced in rabbit
SIGMA/N9528 - whole antiserum
Synonym: NPY Antibody - Anti-Neuropeptide Y (NPY) antibody produced in rabbit; Npy Antibody
MDL Number: MFCD00162717
Product Type: Chemical
ELISA ELISA plates (96 well) were coated with various peptides (Columns 1-7) and BSA (Column 8), (0.5 μg/well), incubated with Anti-Neuropeptide Y (Cat. No. N9528) (antibody dilution 1:5,000) and developed using Goat Anti-Rabbit IgG-Alkaline Phosphatase (Cat. No. A9919) (antibody dilution 1:5,000) and a PNPP substrate (Cat. No. N2770). Columns (left to right) 1. Neuropeptide Y (NPY) (sheep) (Cat. No. N6269) 2. Peptide YY (PYY) (human) (Cat. No. P1306) 3. Neuropeptide Y (NPY) (porcine) (Cat. No. N3266) 4. Neuropeptide Y (NPY) (human) (Cat. No. N5017) 5. Neurokinin B (NKB) (human) (Cat. No. N4143) 6. Neurokinin A (NKA) (human) (Cat. No. N4267) 7. Substance P (SP) (Cat. No. S6883) 8. Albumin, bovine (BSA)
Immunohistochemistry 4% paraformaldehyde perfusion-fixed sections of Rat brain stained with 1:8,000 Anti-Neuropeptide Y (NPY) antibody produced in Rabbit (Cat. No. N9528) followed by Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, sheep, pig, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | : suitable using suitable for IHC on rat brain perfused with 4% paraformaldehyde |
| indirect ELISA: 1:5,000-1:10,000 using using peptide coating at 0.5-1 μg/well in 0.05 M carbonate/bicarbonate buffer, pH 9.6, and pNPP as substrate | |
| UniProt accession no. | P01303  |
| Application: | Anti-Neuropeptide Y (NPY) antibody produced in rabbit has been used in: • single-labeling immunocytochemistry • immunofluorescence • immunostaining • dot blot • radioimmunoassay (RIA) • enzyme-linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | Neuropeptide Y (NPY) plays a vital role in feeding behaviour, anxiety, epilepsy, circadian, rhythm memory processing, sleep, pain and drug addiction. In addition, it is also implicated in regulation of appetite, body weight and obesity. It controls central endocrine secretion and has potent vasoactive effects on the cardiovascular system. NPY is involved in the pathogenesis of atherosclerosis and coronary artery disease. |
| Biochem/physiol Actions: | The antibody recognizes by ELISA, NPY (human), NPY (porcine), NPY (sheep), NPY (13-36), NPY (18-36), and may also cross-reacts with PYY (human). It does not cross-react with neurokinin B (NKB), neurokinin A (NKA), substance P (SP), calcitonin, somatostatin and BSA. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Neuropeptide Y (NPY) is developed in rabbits using synthetic neuropeptide Y (porcine) conjugated to keyhole limpet hemocyanin (KLH) as the immunogen. |
| General description: | Neuropeptide Y (NPY), a 36-amino acid peptide amide, is a major regulatory neuropeptide widely distributed in the mammalian central (CNS) and peripheral nervous systems (PNS). NPY belongs to the pancreatic polypeptide family of peptides which are characterized by a common tertiary structure. Within this family, an intestinal peptide hormone, peptide YY (PYY), is most closely related to NPY. In the CNS, NPY is involved in regulation of blood pressure, memory processing, circadian rhythm, and stimulation of food intake. In the PNS, NPY has potent vasoconstrictor activity and acts as a neurotransmitter/neuromod |
| Immunogen: | synthetic neuropeptide Y (NPY, porcine), conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in human and rat NPY and is highly conserved (84% identity) in the C-terminus region (24-36) of human peptide YY. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |