Monoclonal Anti-Nitric Oxide Synthase, Inducible antibody produced in mouse
SIGMA/N9657 - clone NOS-IN, ascites fluid
Synonym: Anti-iNOS
MDL Number: MFCD00675308
Product Type: Chemical
Immunofluorescence IFN-γ/LPS-activated RAW-264.7 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton™ X-100. Fixed cells were stained with Mouse Anti-Nitric Oxide Synthase (Inducible) Clone: NOS-IN (Cat. No. N9657) diluted to 1:2,000. The antibody was developed using Goat Anti-Mouse IgG, Cy3-conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting IFN-γ/LPS-activated RAW-264.7 cell extract was separated on SDS-PAGE and probed with Mouse Anti-Nitric Oxide Synthase (inducible) Clone: NOS-IN (Cat. No. N9657). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:500 2. Antibody dilution 1:1,000 3. Antibody dilution 1:2,500 4. Antibody dilution 1:5,000
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NOS-IN, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 130 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:1,000 using whole cell extract of the murine macrophage cell line RAW 264.7 activated with LPS and IFN-γ | |
| UniProt accession no. | Q06518  |
| Application: | Monoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS) has been used in • immunofluorescence staining • immunogold electron microscopy • immunoblotting • immunohistochemistry • immunocytochemistry • enzyme linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | Monoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS), antibody reacts specifically with inducible-type nitricoxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain. |
| Biochem/physiol Actions: | Nitric oxide synthase (NOS) is an enzyme involved in the synthesis of nitric oxide (NO), a free radical generated under physiological conditions by virtually all mammalian cells. NO is formed from arginine by NOS which oxidizes a guanidino nitrogen of arginine, releasing NO and citrulline. NO is a messenger molecule mediating diverse functions including vasodilatation, neurotransmission, and antimicrobial and anti-tumor activities. Various types of NOS may serve a variety of diverse biological pathways. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Nitric Oxide Synthase, Inducible (iNOS) (mouse IgG1 isotype) is derived from the NOS-IN hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. Nitric oxide synthase (NOS) is classified into three types based on molecular mass, subcellular location, and Ca2+ dependence. Type I NOS (150-160 kDa) is found in neurons. It is also called as NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca2+-regulated NOS (cNOS). Type II (130 kDa), best characterized in macrophages, also known as macrophage NOS (mNOS) or inducible NOS (iNOS). Type III (135 kDa) is found in endothelial cells. It is called as endothelial NOS (eNOS, or ecNOS). Type II NOS is Ca2+ -independent and is expressed in activated macrophages and some glial cells after stimulation. iNOS is also found in several other cell types including hepatocytes, chondrocytes, endothelial cells and fibroblasts. |
| Immunogen: | synthetic peptide corresponding to amino acids 1126-1144 of nitric oxide synthase (NOS) from mouse macrophage (iNOS, also termed macNOS) conjugated to KLH. The immunogen sequence is highly conserved in rat iNOS and diverges from the human iNOS. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |