NEK7, active, GST tagged human
SIGMA/N9913 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Product Type: Chemical
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| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~63 kDa |
| product line | PRECISIO® Kinase |
| Quality Level | 200  |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 188-254 nmol/min·mg |
| storage temp. | −70°C |
| UniProt accession no. | Q8TDX7  |
| Biochem/physiol Actions: | NEK7 is a member of the NIMA family of serine/threonine kinases. In contrast to the other documented NIMA-related kinases, NEK7 harbor its catalytic domain in the C-terminus of the protein. Immunofluorescence studies suggest that NEK7 is cytoplasmic and located on chromosome 1. The major protein kinase that is active on the p70 S6 kinase hydrophobic regulatory site (FXXFS/TF/Y) Thr412, was purified from rat liver and identified as NEK7.NEK7 kinase activity is rapidly and efficiently increased by serum deprivation, and may be regulated in a cell cycle-dependent manner. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 188-254 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |