Polynucleotide phosphorylase from Synechocystis sp.
SIGMA/N9914 - recombinant, expressed in E. coli
Synonym: PNPase; Polyribonucleotide Nucleotidyltransferase
MDL Number: MFCD00131992
Product Type: Chemical
SDS-PAGE Polynucleotide phosphorylase from Synechocystis sp. (Cat. No. N9914) was assessed on SDS-PAGE.
Unit Definition One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37 °C.
| application(s) | cell analysis |
| assay | 90% (SDS-PAGE) |
| biological source | bacterial ( |
| description | Histidine tagged |
| form | solution |
| mol wt | 85 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| specific activity | ≥500 units/mg protein |
| storage temp. | −70°C |
| suitability | suitable for molecular biology |
| technique(s) | cell based assay: suitable |
| Application: | Polynucleotide phosphorylase has been used in a study to discover that a major function of PNPase is the synthesis of CDP. It has also been used in a study to investigate the enzyme responsible for RNA 3′-tail synthesis in S. coelicolor. |
| Biochem/physiol Actions: | Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity. |
| Biochem/physiol Actions: | Polynucleotide phosphorylase localizes to the intermembrane space of mitochondria and has a critical function in regulating mitochondrial homeostasis in human cells. |
| General description: | Polynuclotide phosphorlyase in spinach chloroplasts acts as a exonuclease and a poly(A) polymerase. |
| Other Notes: | One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37 °C. |
| Other Notes: | Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, 60 mM KCl, 20% (w/v) Glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 90% (SDS-PAGE) |
| activity | specific activity: ≥500 units/mg protein |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 2.7.7.8 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |