GenElute™ HP Plasmid Midiprep Kit
SIGMA/NA0200 - sufficient for 25 purifications
Synonym: GenElute™ HP Plasmid Midiprep Kit; HP midiprep kit; fast midiprep kit; fast plasmid isolation kit; plasmid extraction kit; plasmid isolation kit; plasmid purification kit; ultrafast plasmid isolation kit; Gen Elute
Product Type: Chemical
Electropherogram revealing sequence from pCMV-SPORT-βgal purified from GenElute™ HP Plasmid Midiprep Kit.
Cycle Sequencing was performed using 500 ng template, a T7 sequencing primer and ABI BigDye® terminator chemistry. Sequencing reactions were resolved on an ABI Prism® 377 XL instrument with a 48 cm gel cassette containing 4.5% AutoPAGE™ Plus acrylamide at 2.4 kV, 48 °C for 7 hours.
Cycle Sequencing was performed using 500 ng template, a T7 sequencing primer and ABI BigDye® terminator chemistry. Sequencing reactions were resolved on an ABI Prism® 377 XL instrument with a 48 cm gel cassette containing 4.5% AutoPAGE™ Plus acrylamide at 2.4 kV, 48 °C for 7 hours.
Solutions are drawn through the column by vacuum instead of centrifugation, increasing speed and decreasing hands on time.
GenElute High Performance (HP) Plasmid Kits
Comparison of time required per isolation using different Maxiprep plasmid isolation systems. Plasmid DNA was isolated following each manufacturers recommended protocol. Each system was tested multiple times and the average preparation time is shown here.
Electropherogram revealing >700 bases of sequence from pCMV-SPORT-βgal purified from GenElute™ HP Plasmid Midiprep Kit. Cycle Sequencing was performed using 500 ng template, a T7 sequencing primer and ABI BigDye® terminator chemistry. Sequencing reactions were resolved on an ABI Prism® 377 XL instrument with a 48 cm gel cassette containing 4.5% AutoPAGE Plus acrylamide at 2.4 kV, 48 °C for 7 hours.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category |  , Aligned |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| storage temp. | 15-25°C |
| sustainability | Greener Alternative Product |
| technique(s) | DNA purification: suitable |
| usage | sufficient for 25 purifications |
| Application: | GenElute™ HP Plasmid Midiprep Kit has been used to isolate and purify plasmids. |
| Features and Benefits: | • From harvested bacterial culture to pure plasmid DNA in 30 minutes or less • Up to 350 μg of high-copy plasmid DNA • Flexibility of a vacuum or spin format • No phenol/chloroform extraction or alcohol precipitation required • Contains fewer plastic components, reducing the amount of waste |
| General description: | Click here to view the kit protocol |
| General description: | The GenElute HP Plasmid Midiprep Purification kits offer an ultrafast and efficient solution for large-scale plasmid preparation from E. coli cultures. These kits combine silica-based membrane technology and the convenience of spin or vacuum format. The kits also include a special filter cartridge, which replaces the centrifugation step following alkaline lysis. Following lysis the DNA is bound to the silica membrane and contaminants are removed with a simple wash step. The DNA is then eluted in water or TE buffer by centrifugation. The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | For additional information, please see www.sigma-aldrich.com/gen. |
| Symbol |     GHS02,GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H226 - H290 - H302 - H314 - H334 - H336 |
| Precautionary statements | P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 10-22-36/38-67 |
| Safety Statements | 26 |
| RIDADR | UN 3316 9 |
| Storage Temp. | 15-25°C |
| UNSPSC | 41105501 |
| Components | Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. ); Wash Solution 1; Wash Solution 2; RNase A solution; Column Preparation Solution; Lysis Solution |