GenElute™ HP Endotoxin-Free Plasmid Megaprep Kit
SIGMA/NA0600 - 1 kit sufficient for 5 preparations
Synonym: Gen Elute; GenElute Endotoxin-Free Plasmid Kit
Product Type: Chemical
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category |  , Aligned |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| storage temp. | 15-25°C |
| sustainability | Greener Alternative Product |
| usage | kit sufficient for 5 preparations |
| Application: | GenElute™ HP Endotoxin-Free Plasmid Megaprep Kit has been used to purify plasmid DNA. |
| Application: | High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells. |
| Features and Benefits: | • Up to 5 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg • Only 90 minutes from pelleted cells to purified plasmid • Vacuum format with no ethanol precipitation required |
| General description: | Click here to view the kit protocol |
| General description: | The GenElute HP Endotoxin-Free Plasmid Megaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 5 mg of high copy plasmid DNA with ≤ 0.1 endotoxin units/μg can be recovered from 200 mL - 1.0 L LB medium or 200 - 600 mL TB medium of E. coli culture in less than 1.5 hours. Note that the actual yield depends on the strain, the plasmid, and the culture medium used. High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digest, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | For additional information, please see www.sigma-aldrich.com/gen. |
| Symbol |     GHS02,GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H226 - H290 - H302 + H332 - H315 - H319 - H334 - H336 - H350 - H372 |
| Precautionary statements | P202 - P210 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 10-22-36/37/38-48/20-67 |
| Safety Statements | 26 |
| RIDADR | UN 3316 9 |
| Flash Point(F) | 77.0 °F - closed cup |
| Flash Point(C) | 25 °C - closed cup |
| Storage Temp. | 15-25°C |
| UNSPSC | 41105501 |
| Components | GenElute™ HP Pro Megaprep Binding Columns 5; Column Preparation Solution 2; Neutralization Solution EF; Lysis Solution; E-Toxate™ Water, endotoxin, free; Wash Solution 2; Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. ); RNase A solution; Binding Solution; Wash Solution 1; Red Vac Cap |