GenElute™ HP Select Plasmid Gigaprep Kit
SIGMA/NA0800 - sufficient for 5 preparations
Synonym: HP gigaprep kit; endo free gigaprep kit; endotoxin free giga kit; endotoxin free plasmid kit; fast gigaprep kit; fast plasmid isolation; plasmid extraction kit; plasmid isolation kit; plasmid purification kit; ultrafast plasmid isolation; Gen Elute; GenElute™ HP Plasmid Kit
Product Type: Chemical
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category | Aligned  , |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| storage temp. | 15-25°C |
| sustainability | Greener Alternative Product |
| technique(s) | DNA extraction: suitable |
| usage | sufficient for 5 preparations |
| Application: | GenElute™ HP Select Plasmid Gigaprep Kit has been used to purify plasmids. |
| Application: | High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells and gene therapy research. |
| Features and Benefits: | • Up to 15 mg with endotoxin levels of ≤ 0.1 EU/μg • Only 90 minutes from pelleted cells to purified plasmid • Vacuum format with no ethanol precipitation • Can purify low-, medium-, and high-copy plasmid DNA |
| General description: | Click here to view the kit protocol |
| General description: | The GenElute HP Select Plasmid Gigaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 15 mg of high copy plasmid DNA with ≤0.1 endotoxin unit/μg plasmid DNA can be recovered from E. coli cultures in 1.2-2.5 L Luria Broth (LB) medium or 600 mL-1.2 L of Terrific Broth (TB) medium in less than 2 hours after harvesting the cells. Note that the actual yield depends on the strain, the plasmid, and the culture medium used. An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is cleared by filtration followed by the addition of a binding solution optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured onto a silica membrane in the presence of high salts while endotoxins are prevented from adsorbing to the membrane. Contaminants are removed with three wash steps. Finally, the bound DNA is eluted in endotoxin-free water. The DNA is ready for immediate use in applications such as restriction digest, cloning, sequencing, PCR, and transfection of endotoxin-sensitive cell lines. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | For additional information, please see www.sigma-aldrich.com/gen. |
| Symbol |     GHS02,GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H226 - H290 - H302 - H315 - H319 - H334 - H336 - H372 |
| Precautionary statements | P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 10-22-36/37/38-48/20-67 |
| Safety Statements | 26 |
| RIDADR | UN 2924 8(3) / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | 77.0 °F - closed cup |
| Flash Point(C) | 25 °C - closed cup |
| Storage Temp. | 15-25°C |
| UNSPSC | 41105501 |
| Components | GenElute™ HP Pro Gigaprep Binding Column 5; Column Preparation Solution 3; Lysis Solution; Wash Solution 1; RNase A solution; Endocleaning Solution; Binding Solution; Wash Solution 2; Neutralization Solution EF; Wash Solution 2; Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. ) |