GenElute™ Gel Extraction Kit
SIGMA/NA1111 - sufficient for 70 purifications
Synonym: DNA gel extraction kit; DNA gel isolation kit; DNA gel purification kit; PCR gel clean up kit; PCR gel isolation kit; gel isolation kit; gel purification kit; Rapid purification of linear DNA
Product Type: Chemical
Recovery of 50 bp to 2 kb DNA fragments. 6 μL of the PCR markers (Cat. No. P9577) was electrophoresed on a 1× TBE, 1% agarose gel (Cat. No. A9414) and each fragment was excised and purified with either competitor Q’s, Sigma’s, or competitor P’s gel extraction kit per manufacturer’s recommendations. The gel purified samples were resolved on a 1% agarose gel and the percent recovery was determined via densitometry using a BIO-RAD Flour-S Imager.
| Quality Level | 200  |
| storage temp. | 15-25°C |
| technique(s) | DNA purification: suitable |
| usage | sufficient for 70 purifications |
| Application: | GenElute™ Gel Extraction Kit has been used for the purification of DNA fragments from agarose gels. |
| Application: | The isolated DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling. |
| Biochem/physiol Actions: | The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted by solubilizing slices of an agarose gel. The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer. The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution. Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer. |
| Biochem/physiol Actions: | The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted from slices of an agarose gel and are bound to a silica membrane. Contaminants are removed by a simple spin or vacuum wash. The bound DNA is then eluted. The purified DNA is suitable for a variety of downstream applications, such as automated DNA sequencing, PCR, restriction digestion, cloning, and labeling. |
| Features and Benefits: | • Bind up to 10 μg of DNA • Recoveries up to 80% • Up to 3.5 g can be processed per column • Compatible with both standard and low-melting agarose in TAE or TBE buffer |
| General description: | Click here to view the kit protocol |
| General description: | The GenElute Gel Extraction Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels. This kit can also be used to purify DNA from polyacrylamide gels. Typical recovery of DNA is up to 80%. Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Symbol |   GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H290 - H302 + H332 - H314 - H412 |
| Precautionary statements | P273 - P280 - P301 + P312 + P330 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 + P310 |
| Hazard Codes | C |
| Risk Statements | 20/21/22-32-34-52/53 |
| Safety Statements | 26-36/37/39-45-61 |
| RIDADR | UN 1824 8 / PGIII |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Supplemental Hazard Statements | EUH032 |
| Storage Temp. | 15-25°C |
| UNSPSC | 41105501 |
| Components | Gel Solubilization Solution; Column Preparation Solution; Elution Solution (10 mM Tris-HCl, pH 9.0) |