Nuclei Isolation Kit: Nuclei EZ Prep
SIGMA/NUC101 - sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)
Synonym: Rapid mammalian nuclei isolation kit
Product Type: Chemical
Synthesis of mRNA in Nuclei Isoltaed Using the Nuclei EZ Prep Kit (NUC-101). Nuclei were isolated from Jurkat cells and incubated without (open circles) and with (closed circles) 0.25 μg/ml α-amantin at 25°C in [α-32P]GTP labeling mix. Samples were taken at times indicated, and TCA precipitable counts were determined.
| application(s) | cell analysis |
| foreign activity | nuclease and protease, free |
| packaging | pkg of 1 kit |
| Quality Level | 200  |
| shelf life | 1 yr at 2‑8 °C |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| usage | sufficient for 25 nuclei preparations (~1-10×107 cells/preparation) |
| Application: | Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies. |
| Biochem/physiol Actions: | The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells. |
| General description: | The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types. |
| Other Notes: | All procedures should be carried out on ice or 2-8°C. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |