Monoclonal Anti-Ornithine Decarboxylase (ODC) antibody produced in mouse
SIGMA/O1136 - clone ODC-29, ascites fluid
Synonym: Ornithine Decarboxylase Antibody; Ornithine Decarboxylase Antibody - Monoclonal Anti-Ornithine Decarboxylase (ODC) antibody produced in mouse
MDL Number: MFCD00282394
Product Type: Chemical
Immunoblotting
Lysate from 653-1 mouse myeloma cell line was separated on SDS-PAGE and probed with Monoclonal Anti-Ornithine Decarboxylase, clone ODC-29 (Cat. No. O1136). The antibody was developed with Anti-Mouse IgG (Fab), alkaline phosphatase (Cat. No. A1293) and a NBT/BCIP substrate.
Antibody dilution 1:100.
Lysate from 653-1 mouse myeloma cell line was separated on SDS-PAGE and probed with Monoclonal Anti-Ornithine Decarboxylase, clone ODC-29 (Cat. No. O1136). The antibody was developed with Anti-Mouse IgG (Fab), alkaline phosphatase (Cat. No. A1293) and a NBT/BCIP substrate.
Antibody dilution 1:100.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ODC-29, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2b |
| mol wt | antigen 53 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1:100 | |
| UniProt accession no. | P11926  |
| Application: | Monoclonal Anti-Ornithine Decarboxylase (ODC) antibody produced in mouse has been used in Western blotting. |
| Application: | Monoclonal Anti-Ornithine Decarboxylase (ODC) has also been used in enzyme linked immunosorbent assay (ELISA), immunoprecipitation, immunoblotting and immunohistochemistry. |
| Biochem/physiol Actions: | By immunohistochemical staining of formalin-fixed, paraffin-embedded human prostate and colon carcinoma, the labeling is confined to epithelial cells. |
| Biochem/physiol Actions: | Ornithine Decarboxylase (ODC) biological activity is rapidly induced to promote cell proliferation in response to hormones, drugs, growth factors, mitogens and tumour promoters. ODC mRNA levels are also elevated in transformed cells including lung carcinomas and human colon adenomas and carcinomas. ODC activity in colorectal carcinomas has been shown to be greater than that in adenomas, which, in turn, is greater than that of normal mucosa. ODC has a half-life of 8-30 min, the shortest half-life time reported in eukaryotic cells. |
| Biochem/physiol Actions: | Ornithine decarboxylase (ODC) converts L-ornithine to putrescine. It has a role in cell replication. ODC is also involved in the polyamine synthesis pathway. The protein is upregulated in cancers. Overexpression of ODC has been associated with carcinogenesis in mice. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Ornithine Decarboxylase (ODC) (mouse IgG2b isotype) is derived from the ODC-29 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Its amino acid sequence is well conserved among species; the overall identity of the amino acid sequences of mouse, rat and human ODC proteins is greater than 90%. |
| General description: | Ornithine decarboxylase (ODC) levels increase rapidly after implantation during mouse embryogenesis. The gene encoding this protein is localized on mouse chromosome 12 and human chromosome 2. |
| Immunogen: | recombinant mouse ornithine decarboxylase. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |