Oil Red O solution
SIGMA/O1391 - 0.5% in isopropanol
Synonym: Solvent Red 27; Sudan Red 5B
CAS Number: 1320-06-5
Empirical Formula (Hill Notation): C26H24N4O
Molecular Weight: 408.49
EC Number: 200-661-7
Linear Formula: C26H24N4O
Product Type: Chemical
| εmax | 0.20-0.50 at 510-517 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | dark red |
| concentration | 0.5% in isopropanol |
| form | liquid |
| InChI | 1S/C26H24N4O/c1-16-9-10-1 |
| InChI key | HJAYEODIXUYVIC-LTFAAIOASA |
| Quality Level | 200 ![]() |
| SMILES string | N(N=C3c4c(cccc4)C=CC3= |
| storage temp. | room temp |
| technique(s) | microbe id | staining: suitable |
| Application: | Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue. |
| Application: | Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts. |
| General description: | Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt’s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids. |
| Packaging: | 500 mL in glass bottle |
| Symbol | ![]() GHS02,GHS07 |
| Signal word | Danger |
| Hazard statements | H225 - H319 - H336 |
| Precautionary statements | P210 - P305 + P351 + P338 |
| Hazard Codes | F,Xi |
| Risk Statements | 11-36-67 |
| Safety Statements | 7-16-24/25-26 |
| RIDADR | UN1219 - class 3 - PG 2 - Isopropanol, solution |
| WGK Germany | WGK 2 |
| Flash Point(F) | 53.6 °F - closed cup |
| Flash Point(C) | 12.0 °C - closed cup |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |



