Osteopontin from mouse
SIGMA/O2260 - >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
Synonym: Bone Sialoprotein-1; Early T Lymphocyte Activation Protein-1 (ETA-1); OPN; Secreted Phosphoprotein 1 (Spp1)
MDL Number: MFCD03100698
Product Type: Chemical
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| assay | >95% (SDS-PAGE) |
| biological source | mouse |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | 30 kDa by SDS-PAGE (reducing) |
| 65 kDa by SDS-PAGE (reducing) (doublet) | |
| packaging | pkg of 50 μg |
| Quality Level | 100  |
| recombinant | expressed in NSO cells |
| shipped in | dry ice |
| sterility | sterile |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P10923  |
| Analysis Note: | The biological activity is measured by its ability to mediate 293 cell adhesion. |
| Application: | Osteopontin from mouse has been used: • to study its role in a mouse model of preterm brain injury • as a positive control to analyze the relationship between the vascular calcification process and osteopontin (OPN) overexpression in the neointima of atherosclerotic plaques • to study its interaction on the crystal growth process of hydroxyapatite in agarose hydrogels |
| Biochem/physiol Actions: | Osteopontin (OPN) is a secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig and bovine OPN share 40% amino acid sequence identity. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |