Osteopontin from bovine milk
SIGMA/O3514 - lyophilized powder
Synonym: Bone Sialoprotein-1; Early T Lymphocyte Activation Protein-1 (ETA-1); OPN; Secreted Phosphoprotein 1 (Spp1)
MDL Number: MFCD03100698
Product Type: Chemical
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| biological source | bovine milk |
| form | lyophilized powder |
| mol wt | ~60 kDa |
| packaging | pkg of 50 μg |
| Quality Level | 100  |
| shipped in | ambient |
| sterility | sterile |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P31098  |
| P31096 |
| Analysis Note: | The biological activity is measured by its ability to mediate 293 cell adhesion. |
| Biochem/physiol Actions: | A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity. |
| Biochem/physiol Actions: | Osteopontin stimulates cell mediated responses, chemotaxis of inflammatory cells and anti-inflammatory responses. It binds to av-containing integrins and stimulate cell adhesion and migration. Osteopontin promotes Th1 type immunity. |
| General description: | Osteopontin (OPN) is a multifunctional protein. It is released by macrophages, T cells and epithelial cells. It has 261 amino acid residues with a molecular mass of 29,283 Da. Osteopontin is also called as uropontin. Osteopontin from bovine milk is highly glycosylated and phosphorylated. SPP1 gene (secreted phosphoprotein 1) codes for osteopontin. This gene is located on human chromosome 4 and cow chromosome 6. It has seven exons and six introns. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |