Monoclonal Anti-Opsin antibody produced in mouse
SIGMA/O4886 - clone RET-P1, ascites fluid
Synonym: Anti-Opsin Antibody; Mouse Anti-Opsin; Opsin Detection Antibody
MDL Number: MFCD01865244
Product Type: Chemical
Immunohistochemistry Staining of frozen sections of rat eye with 1:10,000 Monoclonal Anti-Opsin Clone: RET-P1 (Cat. No. O4886) followed by Goat Anti-Mouse IgG (Fab)-FITC (Cat. No. F5262).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | RET-P1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 39 kDa by immunoblotting (IB of rat retina produces closely spaced doublet) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | goldfish, mouse, tiger salamander, rat, amphibian, quail, dove, duck, rabbit, bovine, human, turtle |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | electron microscopy: suitable |
| immunocytochemistry: suitable using cultured cells | |
| immunohistochemistry (frozen sections): 1:10,000 using frozen sections of rat eye | |
| indirect ELISA: suitable | |
| radioimmunoassay: suitable | |
| western blot: suitable | |
| UniProt accession no. | P08100  |
| Application: | Monoclonal Anti-Opsin antibody produced in mouse has been used in immunofluorescence and histology. |
| Application: | Mouse monoclonal clone RET-P1 anti-Opsin antibody is used to tag opsin/rhodopsin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunocytochemistry of cultured cells, immunohistochemistry (paraformaldehyde/glutara |
| Biochem/physiol Actions: | Mutations in rhodopsin gene are linked to retinitis pigmentosa (RP) and congenital night blindness. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Mouse monoclonal clone RET-P1 anti-Opsin antibody recognizes an epitope located in amino acid residues 4-10 at the N-terminus of the rhodopsin molecule. In immunoblotting of rat retina, the antibody labels a closely spaced doublet of 39 kDa and less intense bands of 78 and 115 kDa, representing rhodopsin monomer and aggregates. Higher M.W. bands are stained in pituitary (251 and 288 kDa) and in hypothalamus (269 and 331 kDa) in addition to several lower M.W. bands. The product specifically labels the cell bodies, outer and inner segments (rods but not cones) of rat photoreceptor surface. It reacts with bovine, duck, amphibian, goldfish, rabbit, rat, turtle, tiger salamander, mouse, dove, quail, and human rhodopsin/opsin. |
| General description: | Opsin belongs to rhodopsin receptor. The gene is located on human chromosome 3q22.1. Opsin gene is mainly expressed in rod cells. |
| General description: | Photoreceptors are responsible for the initial step in visual processing, converting the signal of photon absorption into synaptic transmission. The visual pigment in vertebrate rods responsible for the absorption of light quanta is rhodopsin (also called opsin). Rhodopsin comprises >95% of the rod outer segments (ROS) intrinsic membrane protein and is a glycoprotein possessing two asparagine-linked oligosaccharide groups at amino acid residues 2 and 15 of the N-terminus. Light is absorbed by rhodopsin and the subsequent conformational change leads to the activation of a cyclic GMP phosphodiesterase through a specific G-protein intermediate, transducin. |
| Immunogen: | rat retinal membranes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |