Anti-ORAI1 antibody produced in rabbit
SIGMA/O8264 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CRACM1; Anti-Calcium release-activated calcium channel protein 1; Anti-TMEM142A; Anti-Transmembrane protein 142A
Product Type: Chemical
Immunofluorescence
Human Jurkat cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/mL Anti-ORAI1 antibody produced in Rabbit (Cat. No. O8264). The antibody was developed with Goat Anti-Rabbit IgG, Cy3™ conjugate. Pictures B, C: cells were counterstained with DAPI (blue) to stain nuclei.
Human Jurkat cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/mL Anti-ORAI1 antibody produced in Rabbit (Cat. No. O8264). The antibody was developed with Goat Anti-Rabbit IgG, Cy3™ conjugate. Pictures B, C: cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of Human HEK-293T cells expressing ORAI1 was separated on SDS-PAGE and probed with Anti-ORAI1 antibody produced in Rabbit (Cat. No. O8264). The antibody was developed using 1:30,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. 1 μg/mL antibody + inhibition with immunizing peptide 4. 2 μg/mL antibody 5. Negative control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~35 kDa (non-glycosylated) |
| antigen ~50 kDa (glycosylated) | |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using whole extract of human HEK-293T cells expressing ORAI1 |
| UniProt accession no. | Q96D31  |
| Application: | Anti-ORAI1 antibody produced in rabbit has been used in immunohistochemistry and Western blotting. |
| Biochem/physiol Actions: | ORAI calcium release-activated calcium modulator 1 (ORAI1) modulates Ca2+ entry into cells. The channel is activated by stromal interaction molecule-1 (STIM-1). Mutation in the gene encoding the protein has been linked to late-onset tubular aggregate myopathy and congenital miosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | ORAI calcium release-activated calcium modulator 1 (ORAI1) is the pore-forming subunit of the Ca2+ release-activated (CRAC) channel. The protein possesses four predicted transmembrane domains and intracellular amino and carboxyl-terminal domains. The ORAI1 gene is localized on human chromosome 12q24.31. |
| Immunogen: | synthetic peptide corresponding to amino acids 288-301 of human ORAI1, conjugated to KLH. The corresponding sequence differs by 2 amino acids in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |