Anti-PIP5K3 (N-terminal) antibody produced in rabbit

SIGMA/P0054 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-1-phosphatidylinositol-4-phosphate 5-kinase; Anti-Fab1; Anti-Fyve finger-containing phosphoinositide kinase; Anti-P235; Anti-PIKfyve; Anti-PIP5K; Anti-Phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-P0054-200UL	200 µL	$591.00 1/EA	Add To Favorites

Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-PIP5K3 (N-terminal) antibody produced in Rabbit (Cat. No. P0054) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing PIP5K3 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1.5 μg/mL.

Immunoblotting
Cell lysates were separated on SDS-PAGE, probed with 1.5 μg/mL Anti-PIP5K3 (N-terminal) (Cat. No. P0054) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. HEK-293T overexpressing PIP5K3
2. HEK-293T untransfected
3. HEK-293T overexpressing PIP5K3; Antibody + PIP5K3 immunizing peptide (human, 1-16)

Properties
Descriptions
Safety Info.
Basic Data

antibody form	affinity isolated antibody
antibody product type	primary antibodies
biological source	rabbit
clone	polyclonal
concentration	~1.5 mg/mL
conjugate	unconjugated
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation
form	buffered aqueous solution
packaging	antibody small pack of 25 μL
Quality Level	200
shipped in	dry ice
species reactivity	human
storage temp.	−20°C
target post-translational modification	unmodified
technique(s)	western blot: 2-4 μg/mL using HEK-293T cells expressing human PIP5K3.

Application:	Anti-PIP5K3 (N-terminal) antibody produced in rabbit can be used in several immunochemical techniques including immunoblotting.
Biochem/physiol Actions:	Anti-PIP5K3 (N-terminal) specifically recognizes human PIP5K3.
Biochem/physiol Actions:	Phosphatidylinositol-3-phosphate-5-kinase, type 3 (PIP5K3) enzymatic activity is critical for cell morphology and endocytic membrane homeostasis. PIP5K3 acts as a negative regulator of endosome function or regulating endosome-trans-golgi-network (TGN) trafficking. It negatively regulates exocytosis on neurosecretory cells. PIP5K3 regulates the trafficking of IRAP/GLUT4 vesicles to plasma membrane in adipocytes, suggesting that it may play a pleiotropic role in membrane trafficking, including the exocytic pathway.
Biochem/physiol Actions:	Phosphatidylinositol-3-phosphate/phosphatidylinositol-5-kinase, type III (PIP5K3) is involved in the synthesis of phosphatidylinositol 3, 5-bisphosphate. It also possesses a protein kinase activity. PIP5K3 regulates specific signaling pathways as well as membrane trafficking. Mutations in the gene encoding it are associated with fleck corneal dystrophy.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	Phosphatidylinositol-3-phosphate-5-kinase, type 3 (PIP5K3, also known as PIKfyve and FAB1) belongs to a large family of lipid kinases that modify the phosphorylation state of phosphatidylinositol (PtdIns). PIP5K3 has been shown to be localized on early and late endosomes.
General description:	Phosphatidylinositol-3-phosphate/phosphatidylinositol-5-kinase, type III (PIP5K3) is a constituent of a trimolecular protein complex. It is a 2089 amino acid protein. The gene encoding it is located on human chromosome 2 and has 41 exons.
Physical form:	Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

RIDADR	NONH for all modes of transport
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352203

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