Anti-POU5F1 (Oct4) antibody produced in rabbit
SIGMA/P0056 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-OCT3; Anti-OCT4; Anti-OTF3; Anti-OTF4; Anti-Octamer-binding transcription factor 3; Anti-Pou domain, Class 5, Transcription factor 1
Product Type: Chemical
Immunofluorescence Predominant Nuclear localization of POU5F1 (Oct-4) in F9 cells. Fixed cells were stained with 2 μg/mL Rabbit Anti-POU5F1 (Cat. No. P0056) followed by Goat Anti-Rabbit, Cy3 conjugate, and counterstained with DAPI (blue) to stain nuclei (x100 magnification).
Immunoprecipitation Rabbit Anti-POU5F1 (Oct-4) (Cat. No. P0056) was used to immunoprecipitate POU5F1 (Oct-4) from NT2 cell lysate. Detection antibody: Anti-POU5F1(Oct-4) (Cat. No. P0056) Lanes 1. 5 μg antibody. 2. 10 μg antibody. 3. Negative control – without cell lysate 4. Negative control – without IP antibody
Immunoblotting NT2/D1 whole cell extract was separated on SDS-PAGE and probed with 2 μg/mL Rabbit Anti-POU5F1 (Oct4) (Cat. No. P0056). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~43 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using NT2 cell lysate |
| indirect immunofluorescence: 2-5 μg/mL using paraformaldhyde-fixed F9 cells | |
| western blot: 1-2 μg/mL using NT2 cell lysate | |
| UniProt accession no. | Q01860   |
| Application: | Anti-POU5F1 (Oct4) antibody produced in rabbit has been used in Western Blotting. |
| Biochem/physiol Actions: | POU class 5 homeobox 1 (POU5F1) may be a tumor initiating stem-like cell (TSC)-related marker. It has a role in stem cell pluripotency and self-renewal, and also modulates differentiation. The protein has been shown to be expressed in several cancers. POU5F1 is also vital for the induced pluripotent stem cell (iPSC) process. |
| Biochem/physiol Actions: | POU5F1 or Oct4/Oct3 (Octamer-binding transcription factor), functions as an early developmental control gene. It has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and in primordial germ cells. It is down regulated in all differentiated somatic cell types both in vivo and in vitro. This is being accomplished through increased DNA methylation and structural changes involving the immediate upstream regulatory region. POU5F1 serves as a marker for germ cell tumors (GCTs) eliciting pluripotentiality, especially including embryonal, seminoma/ dysgerminoma/ germinoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | POU class 5 homeobox 1 (POU5F1) recognizes defined octamer DNA element by a shared, conserved DNA-binding domain, namely the POU domain. |
| General description: | POU class 5 homeobox 1 (POU5F1), also known as octamer-binding transcription factor-4 (OCT-4), is expressed in embryonic stem cells and germ cells. It belongs to the POU family of transcription factors. Three splice variants of the protein (OCT4A, OCT4B and OCT4B1) have been identified. The gene encoding this transcription factor is localized on human chromosome 6p21.33. |
| Immunogen: | synthetic peptide corresponding to amino acids 19-34 of human POU5F1, conjugated to KLH. The corresponding sequence in rat is 81% homologous. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |