Anti-Peroxiredoxin 6 (C-terminal) antibody produced in rabbit
SIGMA/P0058 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-1-Cys peroxiredoxin; Anti-Acidic calcium-independent phospholipase A2; Anti-Antioxidant protein 2; Anti-Non-selenium glutathione peroxidase, AOP2; Anti-PRDX6
Product Type: Chemical
Immunoprecipitation Rabbit Anti-Peroxiredoxin 6 (C-terminal) (Cat. No. P0058) was used to immunoprecipitate Peroxiredoxin 6 from mouse brain whole extract. Detection antibody: Rabbit Anti-Peroxiredoxin 6 (N-terminal) (Cat. No. P0050). Lanes 1. 2 μg antibody 2. 5 μg antibody 3. Negative control: 5 μg non-relevant antibody 4. Negative control: without precipitating antibody
Immunoblotting Lysates of various cell lines were separated on SDS-PAGE and probed with Rabbit Anti-Peroxiredoxin 6 (C-terminal) (Cat. No. P0058), at a concentration of 1 μg/mL. The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545), and a chemiluminiscence substrate. Lanes 1. A549; 2. HepG2; 3. HeLa; 4. Mouse Brain; 5. Neuro-2a; 6. Rat Brain; 7. PC-12; 8. Rat Liver; 9. Hepa 1-6
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~25 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg using whole extract of mouse brain |
| western blot: 0.5-1.0 μg/mL using whole extract of human HeLa cells. | |
| western blot: 1-2 μg/mL using whole extract of rat brain | |
| UniProt accession no. | P30041  |
| Application: | Anti-Peroxiredoxin 6 (C-terminal) antibody produced in rabbit has been used for Western Blotting. |
| Biochem/physiol Actions: | Peroxiredoxin 6 contains a single redox-active cysteine and uses glutathione to catalyze the reduction of H2O2 and other organic peroxides. Overexpression of peroxiredoxin 6 in cells protects them against oxidative damage, whereas knockdown of this enzyme results in oxidative stress and apoptosis. The phospholipase A2 activity plays an important role in surfactant homeostasis. Peroxiredoxin 6 is a major antioxidant enzyme which functions in antioxidant defense and lung phospholipid metabolism. |
| Biochem/physiol Actions: | PRDX6 (peroxiredoxin 6) has both peroxidase and phospholipase A2 (PLA2) activities and thus, is a bifunctional protein. In liver, as a peroxidase it confers protection against damage from ROS (reactive oxygen species) during ischemia-reperfusion injury. As a PLA2 it is involved in lung surfactant metabolism. It is also essential for normal nicotinamide adenine dinucleotide phosphate-oxidase activity. In the livers of donors after brain death (DBD), this protein suppresses ischemia- and hypoxia-induced liver damage, and its expression is controlled by NF-κB (nuclear factor). In human and mouse neuronal cells, the delivery of PRDX6 protein leads to delay or suppression in oxidative stress-induced neuronal cell death. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | PRDX6 (peroxiredoxin 6) is a mammalian peroxidase belonging to the 1-Cys Prdx family, meaning it has 1-Cys at position 47 (Cys47). This protein is localized to cytoplasm, lysosomes, mitochondria, endoplasmic reticulum (ER) and plasma membrane. It is a homodimeric protein which is especially observed in mammalian lungs.† |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |