Phospholipase D from Streptomyces chromofuscus
SIGMA/P0065 - ≥50,000 units/mL, buffered aqueous glycerol solution
Synonym: Lecithinase D; Phosphatidylcholine phosphatidohydrolase
CAS Number: 9001-87-0
EC Number: 232-639-8
MDL Number: MFCD00131897
Product Type: Chemical
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| form | buffered aqueous glycerol solution |
| mol wt | ~60 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥50,000 units/mL |
| storage temp. | −70°C |
| Application: | Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs). |
| Biochem/physiol Actions: | Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline. PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway. PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis. |
| Biochem/physiol Actions: | Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. |
| General description: | Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues. |
| Other Notes: | One unit will release 1 μmol of choline from |
| Physical form: | Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v). |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ≥50,000 units/mL |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 3.1.4.4 ( BRENDA  | IUBMB ) |
| UNSPSC | 41106300 |