Anti-p62/SQSTM1 (C-terminal) antibody produced in rabbit
SIGMA/P0068 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Sequestosome 1; Anti-
Product Type: Chemical
Immunoprecipitation Rabbit Anti-P62/SQSTM1 (C-Terminal) (Cat. No. P0068) was used to immunoprecipitate P62/SQSTM1 from 3T3 whole cell extract. Detection antibody: Rabbit Anti-P62/SQSTM1(Cat. No. P0067) Lanes 1. 2 μg antibody 2. Negative control: 2 μg non-relevant antibody
Immunoblotting Whole extract of PC12 cells was separated on SDS-PAGE and probed with Rabbit Anti-P62/SQSTM1 (C-Terminal) (Cat. No. P0068). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 2 μg/mL antibody + P62/SQSTM1 immunizing peptide (human, 403-415)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~62 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-4 μg using whole extract of NIH-3T3 cells |
| western blot: 2-4 μg/mL using whole extracts of rat PC12 cells | |
| western blot: 4-8 μg/mL using whole extracts of human A549 cells. | |
| UniProt accession no. | Q13501  |
| Application: | Rabbit polyclonal anti-SQSTM1 antibody has been used: • in immunoblotting • in immunoprecipitation • as a probe to determine the presence and roles of p62 protein/sequestosome 1 in processes such as autophagy |
| Biochem/physiol Actions: | Anti-p62/SQSTM1 (C-terminal) recognizes human, rat, and mouse p62/SQSTM1. |
| Biochem/physiol Actions: | The p62 protein/sequestosome 1 (SQSTM1) is a multifunctional protein, which binds to ubiquitin. It acts as a scaffold protein for the nuclear factor kappa-B (NF-ΚB) signaling pathway. SQSTM1 also regulates apoptosis and autophagy. Mutations in this gene lead to sporadic and familial Paget disease of bone. It is also associated with protein aggregation diseases, such as Lewy bodies in Parkinson′s disease, neurofibrillary tangles in Alzheimer′s disease, and huntingtin aggregates. SQSTM1 is commonly found in inclusion bodies containing polyubiquitinated protein aggregates that accumulate in several degenerative diseases. p62 polymerizes through its N-terminal Phox and Bem1p (PB1) domain and interacts with polyubiquitinated proteins through its C-terminal ubiquitin-associated (UBA) domain. p62 acts as a linker between protein aggregates and the autophagy machinery. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The p62 protein/sequestosome 1 (SQSTM1) comprises a Phox and Bem1p (PB1) domain at the NH2-terminal, a ZZ type zinc finger domain, a Pro, Glu, Ser, and Thr (PEST) region consisting of putative phosphorylation sites, and a ubiquitin-associated (UBA) domain at the COOH-terminal. The SQSTM1 gene is mapped to the human chromosome location 5q35.3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |