Anti-PERK (C-terminal) antibody produced in rabbit
SIGMA/P0074 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-E2AK3; Anti-EIF2AK3; Anti-HsPEK; Anti-PEK; Anti-PRKR-like endoplasmic reticulum kinase; Anti-Pancreatic eIF2α kinase; Anti-WRS
Product Type: Chemical
Immunoblotting Lysate of HEK-293T cells over expressing human PERK was separated on SDS-PAGE and probed with Rabbit Anti-PERK antibodies. The antibodies were developed using Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Anti-PERK (N-terminal) (Cat. No. P0073) dilution 1:500 2. Anti-PERK (C-terminal) (Cat. No. P0074) dilution 1:500
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~150 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human (predicted), mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:500-1:1,000 using whole cell lysate of HEK-293T expressing PERK cells |
| UniProt accession no. | Q9NZJ5  |
| Application: | Anti-PERK (C-terminal) antibody produced in rabbit has been used for western blotting and ERK (extracellular-signal-reg |
| Biochem/physiol Actions: | Anti-PERK C-terminal specifically recognizes PERK. |
| Biochem/physiol Actions: | Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is an endoplasmic reticulum (ER) stress sensor which phosphorylates eukaryotic initiation factor 2 α (eIF2α), thereby reducing the translation of most messenger RNAs (mRNAs). In mice models of frontotemporal dementia, the suppression of translation in a PERK-eIF2α-dependent manner plays a key role in neuronal loss. PERK confers protection to heart against pressure overload-induced congestive heart failure. Mutations in this gene are linked with Wolcott-Rallison syndrome (WRS) which is characterized by permanent neonatal diabetes, exocrine-deficient pancreas, growth retardation and osteopenia. |
| Biochem/physiol Actions: | Protein kinase RNA-like endoplasmic reticulum kinase (PERK) responds to distinct cellular stress signals. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Protein kinase RNA-like endoplasmic reticulum kinase (PERK) belongs to a family of eukaryotic initiation factor 2 α (eIF2α) kinases. It is a transmembrane kinase that is highly expressed in the pancreas. PERK contains two main domains: a kinase domain and an N-terminal domain like inositol- requiring enzyme-1 (IRE1), a protein involved in the unfolded protein response (UPR). |
| Immunogen: | synthetic peptide corresponding to amino acids 1078-1092 of mouse PERK, conjugated to KLH. This sequence is identical in rat and differs by one amino acid in human. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |