Anti-PINK1 (N-terminal) antibody produced in rabbit
SIGMA/P0075 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-BRPK; Anti-PARK6; Anti-PTEN-induced putative kinase 1
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-PINK1 (N-terminal) antibody produced in Rabbit (Cat. No. P0075) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing PINK1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 4 μg/mL.
Immunoblotting Cell lysates of HEK293-T non-transfected (Lane 1), and HEK-293T overexpressing human PINK1 (Lanes 2, 3), were separated on SDS-PAGE, blotted with Anti-PINK1 (N-terminal) (Cat. No. P0075) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody: 4 μg/mL 2. Antibody: 4 μg/mL 3. Antibody: 4 μg/mL+PINK1 immunizing peptide (human, 51-68)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~60 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 4-8 μg/mL using HEK-293T cell lysate expressing human PINK1 |
| UniProt accession no. | Q9BXM7  |
| Application: | Anti-PINK1 (N-terminal) antibody produced in rabbit has been used in immunoblotting. |
| Biochem/physiol Actions: | PINK1 (PTEN induced putative kinase 1) has been found to protect neurons from stress-induced mitochondrial dysfunction and apoptosis. It may protect cells from stress-induced mitochondrial dysfunction. Genetic studies in Drosophila indicate that PINK1 acts upstream of Parkin in a common pathway that influences mitochondrial morphology. PINK1 elicits protection in mouse primary neurons from the dopaminergic neurotoxin 1-methyl-4-phenylpyridine (MPP+)/1-methyl-4-phenyl-1,2,3 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | PINK1 (PTEN induced putative kinase 1, also known as PARK6, BRPK), has been identified as linked to the autosomal recessive form of familial Parkinson′s disease (PD). PINK1 is a Ser/Thr kinase that has been localized to the mitochondria. PINK1 contains an N-terminal mitochondrial targeting motif and a highly conserved kinase domain homologous to Ser/Thr kinases of the Ca2+/calmodulin family. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |