Anti-PINK1 antibody produced in rabbit
SIGMA/P0076 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-BRPK; Anti-PARK6; Anti-PTEN-induced putative kinase protein 1; Anti-
MDL Number: MFCD08064431
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-PINK1 antibody produced in Rabbit (Cat. No. P0076) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing PINK1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1.5 μg/mL.
Immunoblotting Cell lysates of HEK-293T overexpressing human PINK1 (Lanes 1, 3) and HEK293-T non-transfected (Lane 2), were separated on SDS-PAGE, blotted with Anti-PINK1 (Cat. No. P0076) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody: 1.5 μg/mL 2. Antibody: 1.5 μg/mL 3. Antibody: 1.5 μg/mL+PINK1 immunizing peptide (human, 257-274)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~60 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse (predicted), human, rat (predicted) |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using HEK-293T cell lysate expressign human PINK1 |
| UniProt accession no. | Q9BXM7  |
| Application: | Anti-PINK1 antibody produced in rabbit has been used in immunoblotting. |
| Biochem/physiol Actions: | PINK1 (PTEN induced putative kinase 1, also known as PARK6 and BRPK), has been identified as linked to the autosomal recessive form of familial Parkinson disease (PD). PINK1 localized to the mitochondria protects cells from stress-induced mitochondrial dysfunction. Overexpression of wild-type PINK1 has been found to protect neurons from stress-induced mitochondrial dysfunction and apoptosis. Genetic studies in drosophila indicate that PINK1 acts upstream of Parkin in a common pathway that influences mitochondrial morphology. PINK1 activity has been shown to protect primary neurons in mouse from the dopaminergic neurotoxin MPTP (1-methyl-4-phenylpyridin |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | PINK1 is a Ser/Thr kinase that has beenlocalized to the mitochondria, and thought to protectcells from stress-induced mitochondrial dysfunction. |
| General description: | PTEN induced putative kinase 1 (PINK1) is a serine/threonine kinase expressed in mitochondria. It contains an N-terminal mitochondrial targeting motif and a highly conserved kinase domain homologous to serine/threonine kinases of the Ca2+/calmodulin family. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |