Anti-Podoplanin antibody, RAT monoclonal
SIGMA/P0085 - clone NZ-1.2, purified from hybridoma cell culture
Synonym: Anti-AGGRUS; Anti-GP36, glycoprotein, 36-KD; Anti-GP40; Anti-Gp38; Anti-OTS8; Anti-T1A; Anti-T1A-2
Product Type: Chemical
Western Blotting Whole extract of U87 Human Glioblastoma were separated on SDS-PAGE and probed with Anti-Podoplanin antibody, Mouse monoclonal, Clone: NZ-1.2 (Cat. No. P0085). The antibody was developed using 1:10,000 Anti-Rat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A5795). Lanes 1. 4 μg/mL antibody 2. 8 μg/mL antibody 3. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | NZ-1.2, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~36 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 4-8 μg/mL using U87 total cell extract | |
| UniProt accession no. | Q86YL7  |
| Application: | Anti-Podoplanin antibody, Mouse monoclonal has been used in: • immunoblotting • immunoprecipitation • flow cytometry • immunocytochemistry |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) Western Blotting (1 paper) |
| Biochem/physiol Actions: | Expression of podoplanin is regulated by the lymphatic-specific homeobox gene Prox-1 (prospero-1). It is involved in lymphatic vessel formation and might be associated with tissue development and repair. Podoplanin is detected in human angiosarcomas, lymphangiomas, and in Kaposi′s sarcomas. It is implicated in the onset of tumor invasion, metastasis or malignant progression. Podoplanin plays an important role in the maintenance of podocyte foot processes and glomerular permeability. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Podoplanin (rat IgG2a isotype) is derived from the hybridoma NZ-1.2 produced by the fusion of mouse myeloma cells and splenocytes from CD rat immunized with platelet-aggregation-stim |
| Immunogen: | platelet-aggregation-stim |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |