Anti-Prox1 antibody , Mouse monoclonal
SIGMA/P0089 - clone 4G10, purified from hybridoma cell culture
Synonym: Anti-Homeobox prospero-like protein Prox1; Anti-Prospero homeobox 1
MDL Number: MFCD09039204
Product Type: Chemical
Immunofluorescence HepG2 cells were fixed and permeabilized with with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with 2.5 μg/mL Monoclonal Anti-Prox1, Clone: 4G10 (Cat. No. P0089). The antibody was developed using Goat Anti-Mouse IgG, Cy3™-conjugate.
Immunoblotting Whole extract of SH-SY5Y cells was separated on SDS-PAGE and probed with Monoclonal Anti-Prox1, Clone: 4G10 (Cat. No. P0089). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 20 μg/mL antibody 2. Negative Control: without first antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4G10, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~84 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, rat, lizard, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HepG2 cells | |
| western blot: suitable | |
| UniProt accession no. | Q92786  |
| Application: | Anti-Prox1 antibody, Mouse monoclonal has been used in various immunochemical techniques. |
| Biochem/physiol Actions: | Prospero related homeobox gene 1 (Prox1) is a key regulator for neuroblast fate determination and photoreceptor development. Prox1 regulates cell differentiation and organogenesis in lens, retina, lymphatic vessels, liver and pancreas. It was found to act as a tumor suppressor gene, because its deficiency or its mutated forms have been implicated in various human cancers. Knockdown of Prox1 by siRNA or by RNA mutations significantly accelerated the in vitro and in vivo growth of hepatocellular carcinoma (HCC) as well as esophageal cancer cells. Prox1 is hypermethylated and transcriptionally silenced in primary and metastatic breast cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Prox1 (mouse IgG2b isotype) is derived from the hybridoma 4G10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant fusion protein of human Prox1. Prospero related homeobox gene 1 (Prox1) belongs to transcription factor subfamily and is homologous to Drosophila homeobox protein, prospero. |
| Immunogen: | recombinant fusion protein corresponding to amino acids 547-737 of human Prox1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |