PolyFreeze
SIGMA/P0091 - water-soluble support matrix
Synonym: water-soluble support matrix for frozen sectioning
Product Type: Chemical
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| application(s) | hematology histology |
| color | colorless |
| density | 1.030 g/cm3 |
| Quality Level | 200  |
| storage temp. | room temp |
| Application: | PolyFreeze has been used as an embedding medium for frozen sectioning of plant and animal samples. |
| General description: | PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C. Snap freeze using PolyFreeze with isopentane and liquid nitrogen, or dry ice (slush/slurry or bunker). Store specimens frozen in PolyFreeze in liquid nitrogen canisters or in airtight containers in a -80 °C freezer. PolyFreeze does not cause autofluorescence and can easily be washed away during fixation and rinsed prior to staining. Once rinsed, there is no trace of the support matrix to interfere with staining or immunohistochemistry (IHC) reactions. |
| Preparation Note: | PolyFreeze is supplied ready-to-use. A cloudy appearance of the unfrozen product has no effect on its performance. |
| Preparation Note: | Store the product at room temperature |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.030 g/cm3 |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |