Anti-Profilin 2 (C-terminal) antibody produced in rabbit
SIGMA/P0101 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-PFN2
Product Type: Chemical
Immunoblotting Whole extract of SH-SY5Y cells was separated on SDS-PAGE and probed with Rabbit Anti-Profilin 2 (Cat. No. P0101). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1:250 antibody 2. 1:250 antibody + Profilin 2 immunizing peptide (human, 128-140) 3. 1:500 antibody 4. 1:1,000 antibody
Immunoblotting Whole extract of mouse brain was separated on SDS-PAGE and probed with Rabbit Anti-Profilin 2 (Cat. No. P0101). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1:250 antibody 2. 1:250 antibody + Profilin 2 immunizing peptide (human, 128-140) 3. 1:500 antibody 4. 1:1,000 antibody
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~14 kDa |
| packaging | antibody small pack of 25 μL |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:500-1:1,000 using whole extracts of human SH-SY5Y cells, mouse brain, and rat brain |
| UniProt accession no. | P35080   |
| Application: | Anti-Profilin 2 (C-terminal) antibody produced in rabbit has been used in immunostaining and immunoblotting. |
| Biochem/physiol Actions: | Profilins are key regulators of actin dynamics. Profilin 2 (PFN2) associates with a number of proteins involved in membrane trafficking and endocytosis, such as synapsin and dynamin 1. It associates with dynamin 1 through the C-terminal proline-rich domain of dynamin 1 and thereby competes with the binding of SH3 ligands, such as endophilin and amphiphysin, thus interfering with the assembly of the endocytic machinery. Overexpression of profilin 2 inhibits endocytosis. Absence of profilin 2 in neurons increases endocytosis and membrane recycling. Profilin 2 is required for stimulated actin polymerization in the synapse and is involved in the regulation of synaptic vesicle exocytosis, neuronal excitability and complex behavior. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Profilins are actin-monomer-binding proteins, abundantly expressed in all eukaryotic cells from yeast to mammals. Profilin 2 (PFN2) is the neuronal specific isoform. It is an actin-binding protein and is a member of the small G-actin-binding class of protein. |
| Immunogen: | synthetic peptide corresponding to amion acid residues 128-140 of human profilin 2 conjugated to KLH. The corresponding sequence is identical in mouse and rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |