Anti-Prion Protein antibody, Mouse monoclonal
SIGMA/P0110 - clone 8H4, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Prion Protein antibody produced in mouse; Anti-AA960666; Anti-CD230; Anti-PRIP; Anti-PrP; Anti-PrP<C>; Anti-PrPSc; Anti-Prn-i; Anti-RP23-401J24.1; Anti-Sinc
MDL Number: MFCD01865558
Product Type: Chemical
Immunoblotting Mouse Brain extract was separated on SDS-PAGE and probed with 4 μg/mL Monoclonal Anti-Prion Protein Clone: 8H4 (Cat. No. P0110). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8H4, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen 25-35 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, mouse, monkey, human, sheep, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | electron microscopy: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 2-4 μg/mL using mouse brain extract | |
| UniProt accession no. | P04156  |
| Application: | Anti-Prion Protein antibody, mouse monoclonal may be used in: • immunoblotting • flow cytometry • immunocytochemistry • immunoprecipitation • immunoelectron microscopy • immunohistochemistry • enzyme linked immunosorbent assay |
| Biochem/physiol Actions: | Prion-related diseases are fatal neurodegenerative disorders also known as transmissible spongiform encephalopathies (TSEs). Prion plaques are of three types: unicentric (single, compact core), multicentric (two or more cores and definite border), and diffuse plaques without a definite central core. Disease-associated prion protein specifically inhibits the proteolytic β-subunits of the 26S proteasome. This may clarify the mechanism of cell death by the prion protein. Prion protein may be involved in copper utilization, serving to buffer copper at the synaptic cleft or to mediate re-uptake of copper into the presynapse. Alternatively, bound copper may influence PrP binding characteristics. The PrP-copper complex may be crucial for synaptic homeostasis as a result of its antioxidant activity. |
| Biochem/physiol Actions: | The antibody epitope resides within amino acids 145-180 of human prion. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Prion Protein antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma 8H4 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from Prnp-/- mice immunized with recombinant murine PrPc. Prion protein (PrP) is mapped to human chromosome 20p13. PrP is constitutively expressed in brain and other tissues of healthy humans and animals and is present in high levels at the synapse. |
| General description: | Prion is a cell surface glycoprotein present in two isoform- PrPc (a cellular isoform) and PrPsc (a disease associated isoform). Monoclonal anti-prion protein antibody is useful for the treatment of prion disease, by inhibiting the abnormal isoform PrPsc . This antibody can also be used for passive immunization of animals to protect them from prion infection. Anti prion protein antibody may be used to inhibit abnormal prion protein accumulation in cultured scrapie-infected neuroblastoma cells. The antibody reacts specifically with amino acids 145-180 of human prion. It also has specificity for monkey, cow, sheep, deer, squirrel, hamster, mouse and rat. |
| Immunogen: | Recombinant MoPrp residues 121-231 |
| Immunogen: | recombinant mouse PrPC. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |