Phosphatase, Alkaline from bovine intestinal mucosa
SIGMA/P0114 - BioUltra, ≥5,700 DEA units/mg protein
Synonym: Alkaline phosphatase; Orthophosphoric-
CAS Number: 9001-78-9
MDL Number: MFCD00131849
Product Type: Chemical
One DEA unit will hydrolyze 1 micromole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.
| concentration | ≥10 mg/mL |
| form | (40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6) |
| mol wt | dimer ~160 kDa |
| product line | BioUltra |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | ≥5,700 DEA units/mg protein |
| storage temp. | 2-8°C |
| Analysis Note: | Package sizes are based on DEA units |
| Application: | Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. |
| Application: | High specific activity grade recommended for antibody and protein conjugation. |
| Application: | Phosphatase alkaline from bovine intestinal mucosa has been used in a study to measure the hydrolysis kinetics of three polyfluorinated alkyl phosphate monoesters, differing in fluorinated chain length. It has also been used in a study to show that the developmentally controlled expression of the isosomes C-1 and C-2 indicate functional changes in intestinal alkaline phosphatase with tissue maturation. |
| Biochem/physiol Actions: | Alkaline phosphatase was shown to have anti-inflammatory effects and can reduce the severity of septic shock by detoxifying lipid A. |
| Biochem/physiol Actions: | The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids. |
| General description: | Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase. |
| General description: | Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer. |
| General description: | Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity. |
| Other Notes: | One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units) |
| Packaging: | 10000, 50000 units in serum bottle |
| Physical form: | Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥5,700 DEA units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.3.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |