Anti-Purinergic Receptor P2X3 antibody produced in rabbit
SIGMA/P0121 - affinity isolated antibody, lyophilized powder
Product Type: Chemical
Immunohistochemistry Anti-P2X3 Receptor: Cat. No. P0121: Immunohistochemical staining of Rat dorsal root ganglion (DRG) neurons with Anti-P2X3 Receptor antibody, (A). Cells within the DRG were stained (see solid line frame enlarged (B) as well as fibers and the area of entry of dorsal root into spinal cord (see dashed line frame enlarged (C). DAPI is used as the counterstain (in B and C).
Immunoblotting Anti-P2X3 Receptor: Cat. No. P0121: Western blot analysis of Rat DRG lysates. Lanes 1. Anti-P2X3 Receptor antibody, (1:200). 2. Anti-P2X3 Receptor antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable using rat embryo DRG |
| western blot: 1:200 using rat dorsal root ganglion (DRG) lysates | |
| UniProt accession no. | P49654  |
| Application: | Anti-Purinergic Receptor P2X3 antibody has been used in immunohistochemistry. |
| Biochem/physiol Actions: | The P2X3 (purinergic receptor P2X 3) plays a major role in the transmission of pain. Inducing P2X3 generates a desensitizing transient response and initiating P2X2/3 creates a relatively persistent response. P2X3 decreases the sympathetic tone in SH rats. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The P2X3 (purinergic receptor P2X 3) receptor is expressed in primary afferent neurons. It is a subtype of P2X receptors family of ligand-gated ion channels, activated by extracellular ATP (adenosine triphosphate). |
| General description: | The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals. Involvement of ATP in the mechanism of chronic pain contributes to P2X3 receptor as a possible target for the development of pain therapeutics. |
| Immunogen: | peptide (C)VEKQSTDSGAYSIGH corresponding to residues 383-397 of rat P2rx3. |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide. |
| Preparation Note: | Reconstitute the lyophilized vial with 50 μL or 200 μL deionized water, depending on package size. Further dilutions should be made using a carrier protein such as BSA (1-3%). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |