Anti-Paraoxonase 1 (PON1) antibody produced in rabbit
SIGMA/P0123 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Arylesterase; Anti-Coronary Artey Disease, Susceptibility to; Anti-Coronary Spasms, Susceptibility to; Anti-Esterase A (ESA); Anti-Organophosphate Poisoning, Sensitivity to; Anti-PON1; Anti-Parathion Poisoning, Sensitivity to
MDL Number: MFCD09265365
Product Type: Chemical
Immunofluorescence HT-29 Human colon adenocarcinoma cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 5 μg/mL Anti-Paraoxonase 1 (PON1) antibody produced in Rabbit (Cat. No. P0123) followed by 1:600 Anti-Rabbit IgG-Cy3. Picture B: cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of human HT-29 cells was separated on SDS-PAGE and blotted with 2 μg/mL Rabbit Anti-Paraoxonase 1 (PON1) antibody (Cat. No. P0123). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~40 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse (predicted), rat (predicted), human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using human HT-29 cells |
| western blot: 0.5-1 μg/mL using whole extract of human colorectal adenocarcinoma HT-29 cells | |
| UniProt accession no. | P27169  |
| Application: | Anti-Paraoxonase 1 (PON1) antibody produced in rabbit has been used in immunoblotting, and immunofluorescence . |
| Biochem/physiol Actions: | Paraoxonase-1 (PON1) protects lipids from oxidation in lipoproteins, macrophages, and erythrocytes. PON1 may confer protection against coronary artery disease by destroying proinflammatory oxidized lipids present in oxidized low-density lipoproteins (LDLs). PON1 plays a key role in the detoxification of organophosphate insecticides such as parathion, chlorpyrifos and diazinon and nerve gases such as soman and sarin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Paraoxonase-1 (PON1) is a member of serum paraoxonases family, consisting of PON1, PON2, and PON3. Human PONs map to the long arm of chromosome 7. PON1 is polymorphic in human populations, and different individuals express widely different levels of this enzyme. PON1 and PON3 are expressed in the liver and secreted into the blood where they are associated with the high-density lipoprotein (HDL) particle. |
| Immunogen: | synthetic peptide corresponding to amino acids 298-309 of human PON1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence differs by one amino acid in mouse and two amino acids in rat. The peptide sequence differs by one amino acid in human PON2 and two amino acids in human PON3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |