Anti-PKR (ET-20) antibody produced in rabbit
SIGMA/P0244 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-
MDL Number: MFCD04040533
Product Type: Chemical
Western Blotting Whole extract of HeLa cell line induced with interferon-GAMMA were separated on SDS-PAGE and probed with Anti-PKR (ET-20) antibody produced in Rabbit (Cat. No. P0244). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. 1:4,000 antibody 4. 1:2,000 antibody + inhibition with immunizing peptide 5. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 68 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:2,000 using a whole cell extract of the human epitheloid carcinoma HeLa cell line induced with interferon-γ | |
| UniProt accession no. | P19525  |
| Application: | Anti-PKR (ET-20) antibody I suitable for use in western blot (1:2,000 using a whole cell extract of the human epitheloid carcinoma HeLa cell line induced with interferon-γ) and microarray. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | PKR is a double-stranded RNA-dependent serine/threonine kinase that regulates antiproliferative and antiviral functions of interferons. PKR also functions as a translational inhibitor. In genotoxic environments, PKR mediates CDC2 ubiquitination and G2 arrest in cells . Anti-PKR is antibody is specific for human PKR (68 kDa). |
| Immunogen: | C-terminus of human PKR (amino acids 531-550), conjugated to KLH |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |