Calmodulin from bovine heart
SIGMA/P0270 - lyophilized powder, 2,500-10,000 units/mg protein
Synonym: Phosphodiesterase 3′:5′-cyclic nucleotide activator from bovine heart
CAS Number: 77107-46-1
MDL Number: MFCD00131869
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P0270-200UN
| biological source | bovine heart |
| composition | Protein, ≥85% Lowry |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | 2,500-10,000 units/mg protein |
| storage temp. | −20°C |
| technique(s) | calcium flux assay: suitable |
| UniProt accession no. | P62157  |
| Application: | This is the protein activator required for the Ca2+ dependent activation of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520. |
| Biochem/physiol Actions: | Ca2+ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca2+ homeostasis. |
| Other Notes: | One unit will stimulate 0.008 activated unit of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520, in a 3 mL reaction volume at pH 7.5 and 30 °C, to 50% of the maximum activity of the enzyme when saturated with activator, in the presence of 0.01 mM Ca2+. |
| Physical form: | Lyophilized powder containing buffer salts as imidazole and magnesium sulfate |
| Preparation Note: | Purified by a modification of the procedure of Teo, T.S., et al., J. Biol. Chem., 248, 588 (1973). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 2,500-10,000 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |