Anti-Podocin antibody produced in rabbit
SIGMA/P0372 - affinity isolated antibody, buffered aqueous solution
Synonym: Podocin Antibody; Podocin Antibody - Anti-Podocin antibody produced in rabbit
MDL Number: MFCD06798508
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Autophagy attenuates diabetic glomerular damage through protection of hyperglycemia-induced podocyte injury. By: Fang, L., Zhou, Y., et al. in PLoS One, 2013. PubMed ID: 23593240 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0060546), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Candesartan Mediated Amelioration of Cisplatin-Induced Testicular Damage Is Associated with Alterations in Expression Patterns of Nephrin and Podocin. By: Enatsu, N., Miyake, H., et al. in Biomed Res Int, 2015. PubMed ID: 26539476 Image collected and cropped by CiteAb from the following publication, (http://www.hindawi.com/journals/bmri/2015/273784/), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Candesartan Mediated Amelioration of Cisplatin-Induced Testicular Damage Is Associated with Alterations in Expression Patterns of Nephrin and Podocin. By: Enatsu, N., Miyake, H., et al. in Biomed Res Int, 2015. PubMed ID: 26539476 Image collected and cropped by CiteAb from the following publication, (http://www.hindawi.com/journals/bmri/2015/273784/), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Anti-Podocin Antibody: Cat. No. P0372: Immunoperoxidase staining of Podocin in formalin fixed, paraffin-embedded rat kidney at 0.2 μg/mL.
Immunohistochemistry Anti-Podocin Antibody: Cat. No. P0372: Immunoperoxidase staining of Podocin in formalin fixed, paraffin-embedded rat kidney at 0.2 μg/mL.
Immunohistochemistry Anti-Podocin Antibody: Cat. No. P0372: Immunoperoxidase staining of Podocin in formalin fixed, paraffin-embedded mouse kidney at 0.2 μg/mL.
Immunohistochemistry Anti-Podocin Antibody: Cat. No. P0372: Immunoperoxidase staining of Podocin in formalin fixed, paraffin-embedded mouse kidney at 0.2 μg/mL.
Immunoblotting
Extract of rat kidney glomeruli fraction was separated on SDS-PAGE and probed with Anti-Podocin (Cat. No. P0372). The antibody was developed with Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and a ECL substrate.
Lanes
1. Antibody 1.0 μg/mL
2. Antibody 1.0 μg/mL + 5 μg/mL Podocin (aa 367-383) peptide
Extract of rat kidney glomeruli fraction was separated on SDS-PAGE and probed with Anti-Podocin (Cat. No. P0372). The antibody was developed with Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and a ECL substrate.
Lanes
1. Antibody 1.0 μg/mL
2. Antibody 1.0 μg/mL + 5 μg/mL Podocin (aa 367-383) peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~42 kDa (doublet) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 10-20 μg/mL using acetone-fixed human or rat kidney frozen sections |
| western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat glomeruli | |
| UniProt accession no. | Q9NP85  |
| Application: | Anti-Podocin antibody produced in rabbit has been used for Western Blotting and immunohistochemistry. |
| Biochem/physiol Actions: | Anti-Podocin recognizes human, mouse, and rat podocin. |
| Biochem/physiol Actions: | Podocin and nephrin are essential for maintaining the slit diaphragm. Studies show that mutations in this gene account for around 15% cases of nephrotic syndrome cases. Podocin interacts with cholesterol and promotes the formation of protein-lipid supercomplexes, which control ion channel complexes. The podocin gene is known to be associated with autosomal recessive SRNS (nephrotic syndrome type 2). Podocin actively participates in the ultrafiltration process occuring in kidney. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Podocin is a podocyte structural protein, that belongs to the band-7-stomatin family of lipid raft-associated proteins. This family contains more than 1800 conserved proteins which share ∼150 amino acid domains showing similarity to mitochondrial protein prohibitin (PHB). Podocin is a hairpin-like integral membrane protein with intracellular N- and C- termini. Podocin is located at the insertion site of the slit membrane, an intercellular junction found in mammalian kidney. |
| Immunogen: | Synthetic peptide corresponding to amino acid residues of human podocin with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |