Penicillinase from Bacillus cereus
SIGMA/P0389 - lyophilized powder, ≥1500.00 units/mg protein (using benzylpenicillin)
Synonym: β-Lactamase I from Bacillus cereus; β-Lactamase II; Cephalosporinase; Penicillin amido-β-lactam hydrolase
CAS Number: 9073-60-3
MDL Number: MFCD00131819
Product Type: Chemical
Unit Definition Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin. One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 ℃. Stoichiometry: Benzylpenicilloic acid → Penicillin G + H2O
| application(s) | diagnostic assay manufacturing |
| biological source | Bacillus sp. ( |
| composition | Protein, ~10% |
| form | lyophilized powder |
| Quality Level | 300  |
| shipped in | wet ice |
| specific activity | ≥1500.00 units/mg protein (using benzylpenicillin) |
| storage temp. | 2-8°C |
| technique(s) | activity assay: suitable |
| Analysis Note: | Protein determined by biuret. |
| Application: | Penicillinase can be used to reduce levels of penicillin in patients with severe penicillin reactions. |
| Application: | Used in the production of penicillin. |
| Application: | Used in the production of penicillin. Penicillinase from Bacillus cereus has been used in a comparison study with penicillinase from Bacillus subtillis where the Michaelis constant, shape of the Ph/activity curve and immunological properties were shown to be significantly different. |
| Biochem/physiol Actions: | Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin. |
| General description: | Penicillinase from Bacillus cereus has been shown to contain three distinct species when purified and examined for homogeneity by sedimentation analysis, amino-terminal analysis, and vertical acrylamide gel electrophoresis. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units. |
| Physical form: | Lyophilized powder containing phosphate and citrate buffer salts |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1500.00 units/mg protein (using benzylpenicillin) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.5.2.6 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |