Monoclonal Anti-PRMT5 antibody produced in mouse
SIGMA/P0493 - ~2 mg/mL, clone PRMT5-21, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-
MDL Number: MFCD03792429
Product Type: Chemical
Immunoblotting Cell line and tissue lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-PRMT5, Clone: PRMT5-21 (Cat. No. P0493). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Rat brain; 2. Rat heart; 3. HEK-293T; 4. NT2; 5. NIH-3T3; 6. Neuro-2a; 7. PC-12; 8. COS-7; 9. MDBK; 10. MDCK; 11. CHO; 12. SH-SY-5Y; 13. SK-N-SH
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PRMT5-21, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~70 kDa by SDS-PAGE |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, hamster, bovine, canine, monkey, rat, human, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| microarray: suitable | |
| western blot: 2 μg/mL using whole cell extract of human melanoma cell line G361 | |
| UniProt accession no. | O14744  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Monoclonal Anti-PRMT5 antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting. |
| Biochem/physiol Actions: | Protein arginine methyltransferase 5 (PRMT5) participates in the symmetrical dimethylation of arginine residues. Homooligomerization of PRMT5 has an important role in its ability to methylate the myelin basic protein (MBP) protein. PRMT5 also binds the Janus kinases and are involved in an interferon-signaling pathway. It also helps to produce symmetrical ω-NG-dimethylarginine. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-PRMT5 (mouse IgG2a isotype) is derived from the PRMT5-21 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with GST-PRMT5 containing the C-terminal region of the human PRMT5. Protein arginine methyltransferases (PRMT) 5 belongs to the type II arginine methyltransferases. PRMT5 (also known as Skb1Hs/janus kinase-binding protein 1 (JBP1) exists as homo-oligomeric complexes, which includes a dimer and tetramer. PRMT5 is found mainly in the cytoplasm. This gene is located on human chromosome 14q11.2. |
| Immunogen: | GST-PRMT5 containing the C-terminal region of the human PRMT5 (amino acids 315-637). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |