Anti-PMP70 antibody produced in rabbit
SIGMA/P0497 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-70 kDa Peroxisomal membrane Protein; Anti-ABCD3; Anti-PXMP1
MDL Number: MFCD01865555
Product Type: Chemical
Immunofluorescence
Mouse NIH3T3 cells were fixed and permeabilized with 3% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1.5 μg/mL Anti-PMP70 (Cat. No. P0497) and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
Mouse NIH3T3 cells were fixed and permeabilized with 3% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1.5 μg/mL Anti-PMP70 (Cat. No. P0497) and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
Enhanced Validation-By Independent Antibodies Immunofluorescence. 3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton. Fixed cells were stained with 5 μg/mL Anti-PMP70 antibody produced in Rabbit (Cat. No. P0497) (A). The antibody developed using Anti-Rabbit IgG-Atto 448 conjugate antibody, and 5 μg/mL Anti-PMP70 antibody, Mouse monoclonal, Clone: 70-18 (Cat. No. SAB4200181) (B). The antibody was developed using Anti-Mouse IgG, Cy3 conjugate. Figure C- merged image of images A and B. Results Two Anti-PMP70 antibodies, P0497 (A) and SAB4200181 (B), target different regions of PMP70 show similar staining profiles between the two antibodies (C), demonstrating Independent Antibody Verification.
Western Blotting Whole extract of Rat liver were separated on SDS-PAGE and probed with Anti-PMP70 antibody produced in Rabbit (Cat. No. P0497). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. 1 μg/mL antibody + inhibitor 4. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 70 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 1-2 μg using RIPA extract (0.5 mg) from human HepG2 cells |
| indirect immunofluorescence: 4-8 μg/mL using mouse NIH3T3 cells | |
| western blot (chemiluminescent): 0.5-1 μg/mL using whole extracts of rat PC12 cells or rat kidney extract or rat liver extract | |
| UniProt accession no. | P28288  |
| Application: | Anti-PMP70 antibody produced in rabbit has been used in: • immunocytochemistry • immunostaining • immunofluorescence • western blotting • immunoprecipitation |
| Biochem/physiol Actions: | PMP70 participates in the metabolic transport of long and very long fatty acids into peroxisomes. PMP70 interacts and forms a stable complex with the adrenoleukodystrophy protein(ALDP), and also with several other peroxisomal proteins. ATP binding/hydrolysis by PMP70 and ALDL and their phosphorylation are involved in the regulation of fatty acid transport into peroxisomes. Mutations in the PMP70 (PXMP1) gene may cause a subset of Zellweger syndrome, an autosomal recessive disorder and is characterized by defects in import mechanism for peroxisomal matrix enzymes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The 70 kDa peroxisomal membrane protein (PMP70) is a major component of peroxisomal membranes. The peroxisome is a multifunctional single-membrane organelle associated with eukaryotic cells. PMP70 belongs to the ATP binding cassette (ABC) transporter superfamily. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 644-659 of rat PMP70 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in human. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |