Anti-PRL-3 antibody produced in rabbit
SIGMA/P0498 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-PTP4A3; Anti-Phosphatase of Regenerating Liver
MDL Number: MFCD07370459
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human heart tissue sections were stained with 1:500 Anti-PRL-3 antibody produced in Rabbit (Cat. No. P0498) followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of Human HepG2 cells were separated on SDS-PAGE and probed with Anti-PRL-3 antibody produced in Rabbit (Cat. No. P0498). The antibody was developed with 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:2,000 antibody 2. 1:1,000 antibody 3. 1:500 antibody 4. 1,000 antibody + inhibition with immunizing peptide 5. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~25 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:250-1:500 using protease digested tissue sections from human heart by biotin/ExtrAvidin®-peroxidase staining |
| immunoprecipitation (IP): 0.5-1 μL using preparation containing recombinant human PRL-3 | |
| western blot (chemiluminescent): 1:500-1:1,000 using whole extract of human HepG2 cells | |
| UniProt accession no. | O75365  |
| Application: | Anti-PRL-3 antibody produced in rabbit has been used in western blotting and immunohistochemistry. |
| Biochem/physiol Actions: | Anti-PRL-3 recognizes human phosphatase of regenerating live (PRL)-3 and does not cross-react with human PRL-1 and PRL-2. |
| Biochem/physiol Actions: | Phosphatase of regenerating liver (PRL-3) promotes epithelial-mesenchymal transition. It also plays an essential role in cancer cell migration, invasion and metastasis. It dephosphorylates serine, threonine and tyrosine residues. The protein is overexpressed in various cancers. PTP4A3 associates with transcription factors and regulates gene transcription. In addition, it also regulates breast cancer cell proliferation and invasion. Thus, this protein acts as a potent therapeutic target for breast cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Phosphatase of regenerating liver (PRL-3), also known as protein tyrosine phosphatase type IVA, member 3 (PTP4A3) is a dual-specificity phosphatase. It belongs to the phosphatases of regenerating liver family. The PTP4A3 gene is localized on human chromosome 8q24.3. |
| Immunogen: | Synthetic peptide corresponding to amino acid residues of human PRL-3 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse. |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |