Phospholipase D from Arachis hypogaea (peanut)
SIGMA/P0515 - Type II, lyophilized powder, ≥60 units/mg protein
Synonym: Lecithinase D; Phosphatidylcholine phosphatidohydrolase
CAS Number: 9001-87-0
EC Number: 232-639-8
MDL Number: MFCD00131897
Product Type: Chemical
Unit Definition One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.
| biological source | Arachis hypogaea |
| composition | Protein, ~30% |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥60 units/mg protein |
| storage temp. | −20°C |
| type | Type II |
| Analysis Note: | Protein determined using biuret, unless otherwise indicated. |
| Application: | Phospholipase D from Arachis hypogaea (peanut) has been used in the preparation of lipase enzyme solution. It has also been used to determine its influence on human neutrophil respiratory function. |
| Application: | Research has shown ADP-ribosylation factor regulation of phospholipase D is important in the release of nascent secretory vesicles from the trans-Golgi network. It has also been used in a study to investigate stimulation of Na+-Ca2+ exchange activity in canine cardiac sarcolemmal vesicals. |
| Biochem/physiol Actions: | Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. |
| Biochem/physiol Actions: | Phospholipase D is involved in conferring drought susceptibility in peanuts, which increases the risk of aflatoxin contamination. |
| Biochem/physiol Actions: | Phospholipase D (PLD) modulates cell growth, secretion and the actin cytoskeleton. |
| General description: | Phospholipase D is a phospholipid hydrolyzing enzyme and an important component of receptor-mediated signal transduction responses and regulated secretion. |
| General description: | Phospholipase D (PLD) is abundantly found in the eukaryotes and prokaryotes. It is present in all mammalian cells. In mammals, PLD is initiated by various hormones, neurotransmitters, growth factors and cytokines. |
| Other Notes: | One unit will liberate 1.0 μmol of choline from |
| Physical form: | Partially purified, lyophilized powder containing buffer salts |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ≥60 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.4.4 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |