Anti-Potassium Channel SK3 (KCa3, Kcnn3, SKCa3) antibody produced in rabbit
SIGMA/P0608 - affinity isolated antibody, lyophilized powder
Synonym: Anti-KCa2.3; Anti-SK3; Anti-SKCA3; Anti-ZLS3; Anti-hSK3
MDL Number: MFCD02097224
Product Type: Chemical
Immunohistochemistry Anti-KCa2.3 (SK3) (N-term): Cat. No. P0608: Immunohistochemical staining of Mouse dopaminergic neurons using Anti-KCa2.3 (SK3) (N-term) antibody. A. KCa2.3 is detected in substantia nigra pars compacta. B. Tyrosine hydroxylase staining shows dopaminergic neurons. Triangles point at cells with co-localization.
Immunoblotting Anti-KCa2.3 (SK3) (N-term): Cat. No. P0608: Western blot analysis of Rat brain membranes. Lanes 1. Anti-KCa2.3 (SK3) (N-term) antibody, (1:200). 2. Anti-KCa2.3 (SK3) (N-term) antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 100  |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot (chemiluminescent): 1:200 using rat brain membranes |
| UniProt accession no. | Q9UGI6  |
| Application: | Anti-Potassium Channel SK3 (KCa3, KCNN3, SKCa3) antibody produced in rabbit has been used in western blot analysis (1:250) and immunohistochemical staining (1:50). |
| Biochem/physiol Actions: | Anti-Potassium channel SK3 recognizes the SK3 protein in humans and rats by immunoblotting. Potassium channels contribute to maintaining cell volume, membrane potential, neuronal excitability, and the secretion of transmitters, salt, and hormones. It is extremely sensitive to changes in intracellular calcium levels, which are common during action potentials. KCNN3 helps to determine neuronal firing rates via the after-hyperpolarization. It is a migraine candidate susceptibility gene. Aberrations in the KCNN3 gene may trigger cortical spreading depression (CSD) and trigeminal sensitivity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The calcium-activated potassium ion channel (KCNN3) is a voltage-independent small-conductance calcium-activated potassium channel. It is abundantly expressed in the brain and central nervous system. Its predominant expression is seen in the hypothalamus. KCNN3 gene is located on human chromosome 1q21.3. |
| Immunogen: | synthetic peptide corresponding to amino acids 2-21 of human potassium channel SK3. The epitope is highly homologous in rat. |
| Physical form: | Lyophilized at ~0.6 mg/ml from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 0.05% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |