Pepsin−Agarose from porcine gastric mucosa
SIGMA/P0609 - lyophilized powder, ≥30 units/mg dry solid
Synonym: Pepsin A
MDL Number: MFCD00081840
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P0609-10KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P0609-25KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P0609-50KU
| biological source | Porcine gastric mucosa |
| form | lyophilized powder |
| matrix | 4% cross-linked beaded agarose |
| mol wt | 35 kDa |
| Quality Level | 200  |
| specific activity | ≥30 units/mg dry solid |
| storage temp. | −20°C |
| UniProt accession no. | P00791  |
| Application: | Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin. |
| Application: | Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG. |
| Application: | Used to produce F(ab′)2 fragments of antibodies.1 |
| Biochem/physiol Actions: | Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6. |
| General description: | Pepsin is an enzyme found in gastric secretion. |
| Other Notes: | One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.) |
| Packaging: | 10000, 25000, 50000 units in poly bottle |
| Physical form: | Lyophilized powder stabilized with lactose |
| Preparation Note: | One gram solid swells to 5-10ml packed gel. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 - P342 + P311 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22-45-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥30 units/mg dry solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.23.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |