Anti-Potassium Channel Kv11.1 Extracellular antibody produced in rabbit
SIGMA/P0749 - affinity isolated antibody, lyophilized powder
Synonym: Anti-HERG; Anti-KCNH2
MDL Number: MFCD02097212
Product Type: Chemical
Immunohistochemistry Anti-Kv11.1 (HERG) (extracellular): Cat. No. P0749: Immunohistochemical staining of Rat heart using Anti-Kv11.1 (HERG) (extracellular) antibody. A. Transversal section of the atrium wall; note that arterial smooth muscle fibers were not stained (green arrow). B. Longitudinal section of the myocardium. C. Section showing myocardium and endocardium (red arrow). DAB product is brown and the counterstain is cresyl violet.
Immunocytochemistry Anti-Kv11.1 (HERG) (extracellular): Cat. No. P0749: Immunocytochemical staining of live intact HEK-293 transfected cells.Cells were stained with Anti-Kv11.1 (HERG) (extracellular) antibody, (1:25) (left panel) or with Kv11.1 (HERG) (extracellular) antibody preincubated with the control peptide antigen (right panel), followed by Goat Anti-Rabbit-AlexaFluor-555 secondary antibody (red). Nuclei of the living cells were stained with the cell permeable dye Hoechst 33342 (blue). Note that not all cells are stained, indicating that not all the Kv11.1 channels are expressed at the cell membrane at the moment of staining.
Immunoblotting Anti-KV11.1 (HERG) (extracellular): Cat. No. P0749: Western blot analysis of Kv11.1 transfected HEK-293 cells. Lanes 1. Anti-KV11.1 (HERG) (extracellular) antibody, (1:200). 2. Anti-KV11.1 (HERG) (extracellular) antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 2-8 μg using live intact K562 (human chronic myelogenous leukemia) cells (per 106 cells) |
| immunocytochemistry: 1:25 | |
| western blot: 1:200 using HEK-KV11.1 transfected cells | |
| UniProt accession no. | Q12809  |
| Application: | Anti-Potassium channel Kv11.1 extracellular antibodies may be used in immunofluorescence microscopy . It may also be used for the detection of Kv11.1 by techniques such as immunocytochemistry, flow cytometry using live intact K562 (human chronic myelogenous leukemia) cells and western blotting. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The K+ channel family has been sub-grouped in to 2 TM, 4 TM and 6 TM K+ channels based on the number of transmembrane domains. The voltage-gated Potassium or Kv channels belong to the 6 TM K+ channel family that also include other sub-types such as the hERG, KCNQ, and EAG channels. Kv11.1 belongs to the EAG family and facilitates the repolarization of normal action potential in heart muscles. Genetic alterations in Kv11.1 can cause heart disorders such as long QT syndrome, sudden cardiac death and arhythmias. Kv11.1 also regulates cell growth and hence may be studied as a target for cancer treatment. Anti-Potassium channel Kv11.1 extracellular antibodies are specific to Kv11.1. |
| Immunogen: | peptide AFLLKETEEGPPATEC corresponding to residues 430-445 of human KV11.1. This sequence has 11/16 residues identical in mouse and rat and has 15/16 residues identical in rabbit and dog. |
| Physical form: | Lyophilized powder from phosphate buffered saline containing 1% bovine serum albumin and 0.05% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |