Anti-Potassium Channel Kv10.1 (EAG-1) antibody produced in rabbit
SIGMA/P0999 - affinity isolated antibody, lyophilized powder
Synonym: Anti-Ether-a-go-go K+ channel 1; Anti-KCNH1
MDL Number: MFCD08705506
Product Type: Chemical
Immunocytochemistry Anti-Kv10.1 (EAG-1): Cat. No. P0999: Immunocytochemical staining of pA. Cells were stained with Anti-Kv10.1 (EAG-1) antibody, (1:200) followed by Goat Anti-Rabbit-AlexaFluor-555 secondary antibody (red). B. Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue). C. Merged image of panels A and B.
Immunoblotting Anti-KV10.1 (EAG-1): Cat. No. P0999: Western blot analysis of Rat brain lysate (lanes 1,3) and HEK-KV10.1 (lanes 2,4). Lanes 1,2. Anti- KV10.1 (EAG-1) antibody, (1:200). 3,4. Anti-KV10.1 (EAG-1) antibody, preincubated with the control antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:200 using rat brain lysate and transfected cells HEK-KV10.1. |
| UniProt accession no. | O95259  |
| Biochem/physiol Actions: | KCNH1 (potassium voltage-gated channel H1) initiates a slowly activating, non-inactivating K+ current in a heterologous system. In skeletal muscles, it is expressed only during the phase when myoblasts exit the cell cycle and fuse, and thus, it plays an essential role in skeletal muscle development. This protein is found to be expressed in ∼70% human cancers, including gastric, mammary tumors, colon carcinoma, and sarcomas. This channel is implicated in neuronal disorders such as Zimmermann-Laband and Temple-Baraitser syndromes. |
| Biochem/physiol Actions: | Specific for KV10.1 and does not cross react with KV10.2. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Human genome codes for 40 voltage-gated K+ channels (KV). Voltage-gated-like ion channels (VGICs) are the third largest group of signaling molecules found in humans. K+ channels can be divided into four structural types based on their number of transmembrane regions and mode of activation, namely Kir- the inwardly rectifying 2-transmembrane K+ channels, K2P- 2-pore 4-transmembrane K+ channels, KCa- Ca2+-activated 6-transmembrane or 7-transmembrane K+ channels, and KV- voltage-gated 6-transmembrane K+ channels. KCNH1 (potassium voltage-gated channel H1) is a KV channel and is also known as KV10.1. Like all KV members, it is composed of four α-subunits with each containing six transmembrane α-helical segments, and a membrane-re-entering P-loop. These surround a central pore as homotetramers or heterotetramers. KCNH1 mRNA and protein are both abundantly found in the brain, though its peripheral tissue expression is restricted. This gene is localized to human chromosome 1q32.1. |
| General description: | Potassium Channel Kv10.1 (EAG-1) encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming α-subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. |
| Immunogen: | peptide GDPAKRKGWARFKDAC corresponding to residues 802-817 of rat KV10.1. This sequence is identical in mouse and has 14/16 residues identical in human. |
| Physical form: | Lyophilized from phosphate buffered saline containing 1% bovine serum albumin and 0.05% sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |