Phenylalanine Ammonia-Lyase from Rhodotorula glutinis
SIGMA/P1016 - Grade I, buffered aqueous glycerol solution, 0.8-2.0 units/mg protein
Synonym: L-Phenylalanine ammonia-lyase; Phenylalanine Deaminase from Rhodotorula glutinis
CAS Number: 9024-28-6
MDL Number: MFCD00131844
Product Type: Chemical
Unit Definition One unit will deaminate 1.0 μmole of L-phenylalanine to trans-cinnamate and NH3 per min at pH 8.5 at 30°C.
| biological source | fungus (Rhodotorula glutinis) |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | 0.8-2.0 units/mg protein |
| storage temp. | −20°C |
| type | Grade I |
| Analysis Note: | Protein determined by biuret |
| Application: | Phenylalanine ammonia-lyase has been used in a study to assess the effect of heat treatment on lignification of postharvest bamboo shoots. It has also been used in a study to investigate the effect of light on gene expression and podophyllotoxin biosynthesis in Linum album cell culture. |
| Biochem/physiol Actions: | Phenylalanine Ammonia-Lyase from Rhodotorula glutinis can be cleaved and inactivated by the proteases chymotrypsin, subtilisin, and trypsin. |
| Other Notes: | One unit will deaminate 1.0 μmole of |
| Physical form: | Solution in 60% glycerol, 3 mM Tris-HCl, pH 7.5, containing up to 0.5 M (NH4)2SO4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 0.8-2.0 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.3.1.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |