Monoclonal Anti-Paxillin antibody produced in mouse
SIGMA/P1093 - clone PXC-10, ascites fluid
MDL Number: MFCD00212475
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1:200 Monoclonal Anti-Paxillin Clone: PXC-10 (Cat. No. P1093). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-Paxillin Clone: PXC-10 (Cat. No. P1093). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. A431 4. JURKAT 5. NIH-3T3
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PXC-10, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 68-40 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, rat, human, chicken, hamster |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| microarray: suitable | |
| western blot: 1:500 using a cultured human cell line extract | |
| UniProt accession no. | P49023  |
| Application: | Monoclonal Anti-Paxillin antibody produced in mouse has been used in immunoblotting and immunocytochemistry. |
| Biochem/physiol Actions: | Paxillin interacts with several proteins including members of the src family of tyrosine kinases, the transforming protein v-crk, the cytoskeletal protein vinculin, and the tyrosine kinase, focal adhesion kinase (FAK). This interaction has suggested a function for paxillin as a molecular adaptor, responsible for the recruitment of structural and signaling molecules to focal adhesions. |
| Biochem/physiol Actions: | Recognizes an epitope located in the LIM1 domain of the paxillin molecule. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Paxillin (mouse IgG1 isotype) is derived from the PXC-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Paxillin (68 kDa), is a cytoskeletal component. It is found in the ends of actin stress fibers of the focal adhesions, but not in adherens junctions of the cells. The paxillin molecule has a single binding site for vinculin, and at least two binding sites for focal adhesion kinase (FAK), that are separated by an intervening sequence of 100 amino acids. |
| Immunogen: | C-terminal part of recombinant chicken paxillin (amino acids 305-559) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |