Phorbol 12,13-dibutyrate
SIGMA/P1269 - ≥98% (TLC), powder
Synonym: PDBu
CAS Number: 37558-16-0
Empirical Formula (Hill Notation): C28H40O8
Molecular Weight: 504.61
MDL Number: MFCD00036783
Linear Formula: C28H40O8
Product Type: Chemical
| assay | ≥98% (TLC) |
| color | clear light yellow, film |
| form | powder |
| InChI | 1S/C28H40O8/c1-7-9-20(30) |
| InChI key | BQJRUJTZSGYBEZ-YVQNUNKESA |
| Quality Level | 100  |
| SMILES string | CCCC(=O)O[C@@H]1[C@@H](C) |
| solubility | acetone: soluble |
| DMSO: soluble | |
| ethanol: soluble | |
| H2O: 30 μM | |
| storage temp. | −20°C |
| Application: | Phorbol 12,13-dibutyrate has been used as a protein kinase C (PKC) activator to study its effects on human organic anion transporter 4 (hOAT4) activity. It has also been used as a PKC activator to analyze its effects on galectin-3 expression and matrix production in HL-1 cells. |
| Biochem/physiol Actions: | Phorbol 12,13-dibutyrate (PDBu) is a potent activator of protein kinase C (PKC). It is more hydrophilic than phorbol 12-myristate 13-acetate (PMA), which facilitates washing PDBu out of cells in tissue culture. PDBu activates endothelial nitric oxide synthase expression in primary human umbilical vein endothelial cells, which in turn correlates with PKC α and ε expression. |
| Disclaimer: | Photosensitive |
| Features and Benefits: | Less hydrophobic then PMA, making it easier to wash out of cells in tissue culture. |
| Packaging: | 1 mg in ampule |
| Preparation Note: | Stock solution cannot be made in aqueous media; dissolve in a water-miscible organic solvent before dilution to working concentrations in aqueous media. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (TLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352211 |