Poly-L-lysine hydrobromide

SIGMA/P1274 - mol wt 70,000-150,000 by viscosity

Synonym: L-Lysine homopolymer hydrobromide

CAS Number: 25988-63-0
MDL Number: MFCD00237230
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-P1274-25MG	25 mg	$75.30 1/EA	Add To Favorites
45-P1274-100MG	100 mg	$231.00 1/EA	Add To Favorites
45-P1274-500MG	500 mg	$721.00 1/EA	Add To Favorites
45-P1274-1G	1 g	$1170.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: P1274-100MG

Properties
Descriptions
Safety Info.
Basic Data

color	white to off-white
form	solid
InChI	1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
InChI key	WBSCNDJQPKSPII-KKUMJFAQSA-N
mol wt	70,000-150,000 by viscosity
Quality Level	300
SMILES string	Cl.NCCCCC(N)C(O)=O
storage temp.	−20°C
technique(s)	cell culture \| mammalian: suitable

Analysis Note:	Molecular weight based on viscosity and is also assayed by MALLS.
Application:	Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule. Poly-L-lysine hydrobromide is used for the following applications: • Used for some experiments with REF-52 cells, for coating • For electrophysiological experiments, cells were seeded on poly-L-lysine hydrobromide coated or uncoated cover-slips • Cell culture of rat neurons • Indirect binding of monoclonal antibody via poly-llysine
Biochem/physiol Actions:	Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.
Disclaimer:	Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.
Other Notes:	Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.
Packaging:	1 g in poly bottle
Packaging:	25, 100, 500 mg in poly bottle
Preparation Note:	This product has a molecular weight of 70,000-150,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm² of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352209

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