Prehybridization Solution
SIGMA/P1415 - for Northern and Southern blotting, liquid (2x concentrate)
Product Type: Chemical
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| foreign activity | DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected |
| form | liquid (2x concentrate) |
| Quality Level | 100  |
| 200 |
|
| storage temp. | −20°C |
| Application: | Suitable for use as a pre-hybridization buffer in Northern and Southern blot protocols. |
| General description: | Sigma’s Prehybridization Solution is a 2x ready-to-use buffer for Northern and Southern blot protocols. |
| Preparation Note: | Sigma’s 2X Prehybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt’s solutions: • 200 μg/mL DNA • 2mg Bovine Serum Albumin (BSA) • 2mg Ficoll • 2mg Polyvinylpyrrolidone (PVP) • 0.15 M sodium citrate • 1.5M NaCl |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161700 |