Calmodulin from bovine testes
SIGMA/P1431 - BioUltra, ≥98% (SDS-PAGE), lyophilized powder, essentially salt free
Synonym: CaM; Phosphodiesterase 3′:5′-cyclic nucleotide activator
CAS Number: 77107-46-1
MDL Number: MFCD00131869
Product Type: Chemical
Calmodulin exists as a long dumbbell shaped molecule. Each globular end contains two calcium binding domains. Upon binding calcium, calmodulin undergoes a conformational change in which the hydrophobic regions become exposed. These hydrophobic regions are believed to be involved with enzyme binding.
Three lots of BioUltra Calmodulin samples were analyzed by UPLC-UV-MS and deconvoluted accurate mass spectra were generated from the calmodulin peak in each sample: all exhibited intact accurate mass of 16791 Da which is in direct agreement with literature considering an acetylated N-terminus and trimethyllysine at residue 115. UV and MS purity for all lots was >99% based on the UPLC-UV214 and UPLC-MS-TIC chromatograms.
Three lots of BioUltra Calmodulin samples were digested with trypsin and chymotrypsin then analyzed by UPLC-MS-MS. MS-MS data was searched using the MASCOT algorithm with sequence coverage of 83% which included the acetylated N-terminus and the trimethyllysine at residue 115.
SDS-PAGE (4-20%) of calmodulin (Cat. No. P1431) under reducing conditions and stained with Coomassie blue.
| application(s) | cell analysis |
| assay | ≥98% (SDS-PAGE) |
| biological source | bovine testis |
| form | lyophilized powder |
| impurities | salt, essentially free |
| mol wt | 16.79 kDa |
| product line | BioUltra |
| Quality Level | 200  |
| storage condition | (Keep container tightly closed in a dry and well-ventilated place) |
| storage temp. | −20°C |
| technique(s) | ligand binding assay: suitable |
| UniProt accession no. | P62157  |
| Application: | Calmodulin from bovine testes has been used: • as a component of the reaction mixture in PhosphoSens assay to measure Ca2+/calmodulin-dependent protein kinase II α (CaMKIIα) substrate phosphorylation • to generate standard curve for the determination of in situ calmodulin concentration in tissues • as a ligand in radio-ligand binding for studying calmodulin affinity |
| Biochem/physiol Actions: | Ca2+ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca2+ homeostasis. |
| Biochem/physiol Actions: | Calmodulin (CaM) aids in the Ca2+ signal transduction pathway in higher plants and animals. Ca2+ binding is required for CaM activation. Upon activation, CaM binds and activates numerous target proteins involved in a variety of cellular processes including regulation of plant metabolism, phytohormone signaling, ion transport, protein folding, protein phosphorylation and dephosphorylation, cell motility, exocytosis, and cytoskeletal assembly. In neurons, calcium-activated CaM helps in the regulation of glutamate receptors, modulation of proteins in signaling pathways, and regulation of voltage-gated calcium channels (VGCCs) activity. |
| General description: | Research area: Cell Signaling Calmodulin (CaM) is a Ca2+-sensor protein containing four EF-hand motifs that bind to four Ca2+ ions. It is found ubiquitously in all eukaryotes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |